Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential

Table 3 Effects of holding in EH medium at controlled room temperature (25°C) on mitochondrial distribution pattern of equine oocytes examined before or after IVM

Treatment	Nuclear chromatin configuration	n. of analyzed oocytes	Small mitochondrial aggregates (SA) n. (%)	Perinuclear/pericortical (P/P) n. (%)	Abnormal n. (%)
EH	FN	3	3 (100)	0 (0)	0 (0)
	F/I	4	4 (100)	0 (0)	0 (0)
	CC	22	12 (55)	8 (36)	2 (9)
	PI/MI	0	0 (0)	0 (0)	0 (0)
	MII	0	0 (0)	0 (0)	0 (0)
IMM	FN	2	2 (100)	0 (0)	0 (0)
	F/I	9	4 (44)	5 (56)	0 (0)
	CC	20	6 (30)	14 (70)	0 (0)
	PI/MI	3	0 (0)	3 (100)	0 (0)
	MII	2	0 (0)	2 (100)	0 (0)

Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII).
Chi square test with the Yates correction: between treatments and chromatin configurations: NS.

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