Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential

Table 2 Effects of holding in EH medium on energy/redox parameters of equine oocytes examined before or after IVM

Treatment	Nuclear chromatin configuration	n. of analyzed oocytes	Mitochondrial activity	Intracellular ROS levels	Mitochondria/ROS colocalization
EH	FN	4	387.42 ± 347.10^a	661.20 ± 404.12	0.40 ± 0.13^a
IMM	FN	9	1248.63 ± 657.93^b,*	1330.73 ± 651.51	0.64 ± 0.18^b
EH	F/I	2	§	§	§
IMM	F/I	13	273.23 ± 142.95	709.72 ± 423.16	0.56 ± 0.23
EH	CC	20	545.15 ± 283.98	829.89 ± 336.91	0.63 ± 0.16
IMM	CC	12	509.19 ± 242.27**	882.61 ± 486.82	0.67 ± 0.14
EH	PI/MI	11	653.80 ± 191.53	946.64 ± 561.87	0.71 ± 0.11
IMM	PI/MI	0	§	§	§
EH -IVM	POST-IVM MII	32	1112.45 ± 494.10	1434.54 ± 686.00	0.76 ± 0.14
IMM-IVM		43	1372.64 ± 481.34	1982.04 ± 762.74	0.84 ± 0.13

Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII). Mitochondrial activity and intracellular ROS levels are presented as MitoTracker and DCF fluorescence intensities expressed in Arbitrary Densitometric Units (ADU). Mitochondria/ROS colocalization is expressed as Pearson’s correlation coefficient. One-way ANOVA followed by Multiple Comparison Dunn’s method: ^a,b P <0.05; *, **P =0.004; § values for categories with fewer than 3 oocytes are not shown.

ISSN: 1477-7827