Evidence of ERalpha and ERbeta selectivity and partial estrogen agonism in traditional Chinese medicine

Table 5 The EC ₅₀ and maximal transcriptional activity of the traditional Chinese medicine (TCM) and its 18 components that were determined in the HELN-ERα and HELN-ERβ cell lines

		ERα		ERβ
		Max. luciferase activity %	EC₅₀%	Max. luciferase activity %	EC₅₀%
Traditional Chinese medicine		107.4	0.035	78	0.015
Huang Jing	A	48		51
Huang Qi	B	80	0.2	100	0.055
Gu Sai Bu	C	34		23
Ru Xiang	D	38		23
Lu Rong	E	74		33
Ji Nei Jin	F	94	0.2	86.5	0.03
Bai Ji Li	G	44		33
Mai Men Dong	H	93		38
Mo Yao	I	43	0.009	37	0.004
Shu Di	J	78	0.04	61	0.02
Shan yao	K	73		45
Shan Zu Yu	L	69	0.3	49	0.4
Chai Hu	M	57	0.1	43	0.1
Ze Xie	N	33		20
Shen Qu	O	100		61
Gan Cao	P	111	0.004	92	0.002
Fu Ling	Q	108		45
Chen Pi	R	85	0.07	35	0.035

The EC₅₀ values of the herbal medicine and the 18 components are expressed as percentages and correspond to dilutions of the TCM or component (1% = 100-fold dilution). The percentage of maximum luciferase activity (100%) was determined when the cells were exposed to 10^-8M E₂. The complete identification code of the 18 herbal components is explained in Table 2.

ISSN: 1477-7827