Figure 6
The proliferative response of MELN cells to the traditional Chinese medicine (TCM). The proliferation curves were generated by exposing HELN cells to increasing concentrations of E2 (10-13M – 10-8 M) and increasing dilutions of the TCM (1% - 0.001%) for 10 days, and then to the vital mitochondrial dye, 3-[4,5-dimethyliazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), for four hours at 37°C in a 5% CO2 humidified atmosphere. The data are expressed as a percentage of the maximum proliferation (100%), which was defined as the amount of proliferation when the cells were exposed to 10-8M 17β-estradiol. Each value at each concentration or dilution in the curves is the mean of quadruplicate determinations ± standard deviation from three separate experiments.