Table 1 Oligonucleotides sequences used for RT-PCR
Target mRNA | Prime | Sequence |
|---|---|---|
GAPDH | Forward | 5′-GTGGAGATTGTTGCCATCAACG −3′ |
Reverse | 5′-CAGTGGATGCAGGGATGATGTTCTG −3′ | |
StAR | Forward | 5′-GAAGGCTGGAAGAAGGAAAG-3′ |
Reverse | 5′-GAACTCTATCTGGGTCTGTG-3′ | |
CYP11A1 | Forward | 5′- CTGCCTGGGATGTGATTT −3′ |
Reverse | 5′-GGAAGTGCGTGGTGTTTT −3′ | |
3β-HSD | Forward | 5′- TCAAATCCATACCCATACAGC-3′ |
Reverse | 5′- GCCACATTGCCTACATACACT −3′ | |
CYP21A1 | Forward | 5′-ACCTTCCGGTCTACCTGTTT-3′ |
Reverse | 5′-AAGTGCTGTCCTGCTTGTCT-3′ | |
CYP11B1 | Forward | 5′-CTGCTGGGACATTCGTCA-3′ |
Reverse | 5′-ACAGGCCGGAAAGTAAGG-3′ | |
11β-HSD2 | Forward | 5′-TCTCCAGTGGTAACTTTCCG-3′ |
Reverse | 5′-TGCTCAATGTAGTCTTCACC-3′ |