Reproductive Biology and Endocrinology

Table 2 Effects of α-Amanitin treatment on mRNA abundance in bovine 8C embryos

From: Temporal regulation of mRNAs for select bone morphogenetic proteins (BMP), BMP receptors and their associated SMAD proteins during bovine early embryonic development: effects of exogenous BMP2 on embryo developmental progression

Gene	RT with oligo dT		RT with random hexamers
	Control	α-Amanitin treated	Control	α-Amanitin treated
SMAD1	1.15 ± 0.27^a	9.08 ± 1.19^b	1.03 ± 0.16	1.77 ± 0.17
SMAD5	1.17 ± 0.35^a	7.33 ± 1.27^b	1.02 ± 0.12	1.45 ± 0.17
BMP2	1.10 ± 0.27^a	3.19 ± 0.76^b	1.25 ± 0.49	1.30 ± 0.21
BMP3	1.02 ± 0.11^a	2.85 ± 0.60^b	1.15 ± 0.36	3.26 ± 0.88
BMP7	1.02 ± 0.12^a	3.36 ± 0.66^b	1.17 ± 0.40	2.96 ± 1.31
BMP10	1.04 ± 0.19^a	3.82 ± 0.25^b	1.07 ± 0.23	1.59 ± 0.31
ALK3	1.23 ± 0.37^a	9.41 ± 0.72^b	1.06 ± 0.22	1.42 ± 0.31
ALK6	1.12 ± 0.25^a	5.43 ± 0.71^b	1.05 ± 0.20	1.38 ± 0.16
ALK2	1.27 ± 0.50^a	5.73 ± 1.34^b	1.02 ± 0.13	0.86 ± 0.09

Note. Different letters (a and b) represent significantly different value (P≤0.05).

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