Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles

Nina Desai; Stephanie Ploskonka; Linnea R Goodman; Cynthia Austin; Jeffrey Goldberg; Tommaso Falcone

doi:10.1186/1477-7827-12-54

Table 3 Embryos kinetics during development to day 5

From: Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles

	Blasts transferred (BL-T) Mean ± SD	95% CI	Blasts frozen (BL-F) Mean ± SD	95% CI	PQ BL/Non blasts (PQ-D) Mean ± SD	95% CI	p
tPNf	24.8 ± 2.6	24.3- 25.3	25.2 ± 3.0	24.9 - 25.6	26.8 ± 8.3	26.6 - 28.0	0.001
t2	27.2 ± 3.6	26.5 - 27.8	27.7 ± 4.0	27.1-28.2	30.0 ± 8.8	28.8- 31.2	<0.0001
t3	37.6 ± 5.5	36.5-38.7	38.0 ± 5.8	37.4 - 38.6	38 ± 11.8	36.4- 39.6	ns
t4	40.0 ± 5.4	39.0 - 41.1	40.9 ± 6.1	40.3- 41.6	43.4 ± 1 4.3	41.4 - 45.4	0.003
t5	52.0 ± 6.3	50.8 - 53.2	52.4 ± 9.0	51.5 - 53.4	50.2 ± 15.3	48.0 - 52.4	ns
t8	62.1 ± 9.8	60.2- 64.0	63.8 ± 11.7	62.6- 65.1	71.1 ± 21.2	67.8 - 74.3	<0.0001
t9+	73.5 ± 10.3	71.4-75.6	74.7 ± 13.3	73.2-76.3	83.7 ± 24.5	79.7-87.8	<0.0001
tMor	93.9 ± 9.8	91.9 - 95.9	98.2 ± 12.8	96.8 - 99.7	110.7 ± 15.3	106.7 - 114.7	<0.0001
tSB	100.2 ± 7.4	99.0– 101.5	105.5 ± 10.3	104.5 – 106.5	113.1 ± 13.1	110.2 – 116.1	<0.001
tBL	105.2 ± 6.3	103.8 - 106.6	111.1 ± 10.5	109.9 - 112.3	121.4 ± 9.1	116-126.0	<0.0001
tEBL	110.0 ± 5.6	108.6 - 111.4	118.9 ± 12.1	117.3 - 120.4	133.1 ± 9.0	127.1-139.2	<0.0001
cc2 (t3-t2)	10.4 ± 4.5	9.6- 11.3	10.2 ± 4.7	9.7- 10.8	8.6 ± 10.7	7.1 - 10.1	0.02
cc3 (t5-t3)	14.4 ± 5.4	13.4-15.4	14.4 ± 7.3	13.6-15.1	14.0 ± 13.1	12.1-15.8	ns
S1 (t2-tPNf)	2.4 ± 1.7	2.0 – 2.7	2.6 ± 1.9	2.4 -2.8	3.2 ± 3.9	2.6 – 3.7	0.03
s2 (t4-t3)	2.4 ± 4.7	1.5 - 3.4	2.9 ± 5.0	2.4 -3.5	5.3 ± 8.9	4.1- 6.6	<0.0001
s3 (t8-t5)	10.2 ± 8.7	8.4- 11.9	11.5 ± 10.2	10.4 -12.6	21.8 ± 19.1	18.9 - 24.8	<0.0001
t4int (t5-t4)	12.0 ± 5.7	10.9-13.1	11.5 ± 6.5	10.8-12.2	8.7 ± 10.2	7.2-10.1	<0.0001
t5-t2	24.9 ± 5.8	23.8-26.0	24.6 ± 7.8	23.8-25.4	21.9 ± 16.2	19.7-24.2	<0.01

Embryos being cultured for blastocyst transfer cycles (n = 648) were divided into three groups: (A) BL-T, blastocysts transferred, (B) BL-F, blastocysts.
frozen and (C) PQ-D, Poor quality blastocysts/embryos failing to blastulate. Mean timings in BL-T and BL-F blastocysts(Group A, B) shown and compared to Group C. P value <0.05 was considered to be significant.

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