Separation of the male and female Asian seabass gonads under the hierarchical clustering map. The expression profiles of 36 sex-related genes with p-value (gonad type) < 0.01 under a one-way ANOVA analysis were used to generate the hierarchical clustering map. Male and female gonads were clustered into two different clades. Within the female gonad clade (top section), F3 and F4 ovaries were further divided into two sub-clades, which indicated that their gene expression profiles were different. Red boxes indicate high expression, whereas blue boxes indicate low expression. White boxes within the clustering map indicate missing values for a particular sample and gene.