Table 2 List of primary antibodies and isotype controls used for immunohistochemistry

COX2

clone 33

BD Pharmingen Heidelberg Germany

anti-rat COX-2 IgG

1:100

mouse monoclonal

PGT

(G-17) Sc-103085

Santa Cruz Biotechnology CA, USA

IgG against human PGT

1:100

goat polyclonal

PGFS/AKR1C3

custom made canine-specific antibody (Gram et al., 2013 [17])

Eurogentec Seraing Belgium

IgG against canine-specific peptide sequence DTLFATHPDYPFNDED, C-terminal amino acids 309–324

1:750

guinea pig polyclonal

PGES

custom made canine-specific antibody (Kautz et al., 2014 [26])

Eurogentec Seraing Belgium

IgG against canine-specific peptide sequence RSDQDVDRCLRAHRND, C-terminal amino acids 61–76

1:300

guinea pig polyclonal

HPGD

custom made canine-specific antibody (Gram et al., 2013 [17])

Eurogentec Seraing Belgium

IgG against canine-specific peptide sequence HFQDYETTPFHAKTQ, C-terminal amino acids 252–266

1:750

guinea pig polyclonal

EP2

catalog no. 101770

Cayman Chemicals MI, USA

IgG against human EP2, C-terminal amino acids 335–358

1:200

rabbit polyclonal

EP4

catalog no. 101775

Cayman Chemicals MI, USA

IgG against human EP4, C-terminal amino acids 459–488

1:100

rabbit polyclonal

Isotype control

IgG

Vector Laboratories Inc., Burlingame, CA, USA

−

Same protein concentration as primary antibody

mouse

Isotype control

IgG

Vector Laboratories Inc., Burlingame, CA, USA

−

Same protein concentration as primary antibody

rabbit

Isotype control

IgG

Vector Laboratories Inc., Burlingame, CA, USA

−

Same protein concentration as primary antibody

goat

Isotype control

IgG

Vector Laboratories Inc., Burlingame, CA, USA

−

Same protein concentration as primary antibody

guinea pig