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Table 2 List of primary antibodies and isotype controls used for immunohistochemistry

From: Interplacental uterine expression of genes involved in prostaglandin synthesis during canine pregnancy and at induced prepartum luteolysis/abortion

Name/antigen Clone Company Immunogen Concentration Species/type
COX2 clone 33 BD Pharmingen Heidelberg Germany anti-rat COX-2 IgG 1:100 mouse monoclonal
PGT (G-17) Sc-103085 Santa Cruz Biotechnology CA, USA IgG against human PGT 1:100 goat polyclonal
PGFS/AKR1C3 custom made canine-specific antibody (Gram et al., 2013 [17]) Eurogentec Seraing Belgium IgG against canine-specific peptide sequence DTLFATHPDYPFNDED, C-terminal amino acids 309–324 1:750 guinea pig polyclonal
PGES custom made canine-specific antibody (Kautz et al., 2014 [26]) Eurogentec Seraing Belgium IgG against canine-specific peptide sequence RSDQDVDRCLRAHRND, C-terminal amino acids 61–76 1:300 guinea pig polyclonal
HPGD custom made canine-specific antibody (Gram et al., 2013 [17]) Eurogentec Seraing Belgium IgG against canine-specific peptide sequence HFQDYETTPFHAKTQ, C-terminal amino acids 252–266 1:750 guinea pig polyclonal
EP2 catalog no. 101770 Cayman Chemicals MI, USA IgG against human EP2, C-terminal amino acids 335–358 1:200 rabbit polyclonal
EP4 catalog no. 101775 Cayman Chemicals MI, USA IgG against human EP4, C-terminal amino acids 459–488 1:100 rabbit polyclonal
Isotype control IgG Vector Laboratories Inc., Burlingame, CA, USA Same protein concentration as primary antibody mouse
Isotype control IgG Vector Laboratories Inc., Burlingame, CA, USA Same protein concentration as primary antibody rabbit
Isotype control IgG Vector Laboratories Inc., Burlingame, CA, USA Same protein concentration as primary antibody goat
Isotype control IgG Vector Laboratories Inc., Burlingame, CA, USA Same protein concentration as primary antibody guinea pig