Figure 6

The effects of embryo density in media on growth rates. Hybrid strain and B6 mouse zygotes were cultured individually or in groups of 10 embryos in 10 μL sIVF media under 5% O₂ condition. (A) the rates of blastocyst formation at 96 and 116 h post-hCG, and hatching blastocysts, (B) the number of the cells in each blastocyst and (C) the number of cells with fragmented nuclei were analysed. Each culture group had at least 60 hybrid strain embryos and 50 B6 embryos from 3 independent replicates. Statistically significant effects are shown on the graphs. DENSITY and STRAIN were the dependent variables, and INT (abbreviation of interaction) was the interaction effect between DENSITY and STRAIN.