Figure 4

The effects of supplementation of media with mixed amino acids on growth rate of zygotes. Hybrid strain zygotes cultured in groups of 10 in 10 μL GE-HTF or sIVF media under the 5% oxygen tension. The developmental outcomes were assessed at 96 h for blastocyst formation and 116 h for the rates of blastocyst formation and hatching, and the number of the cells accumulated in the resultant blastocyst. At least 100 embryos for each medium from 3 independent replicates were tested. *P < 0.001, compared to embryos cultured in sIVF media.