Figure 1From: Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitroThe effects of embryo culture on developmental viability. (A) Hybrid strain (B6CBF1) zygotes collected 20 h post-hCG or (B) 2-cell embryos 44 h post-hCG were cultured as 10 embryos in a drop of 10 μL GE-HTF medium or sIVF media under 5% CO2 in air (20% O2). The cell number of the cultured embryos and the embryos freshly collected from reproductive tracts at a 12 h interval from (A) 36 h post-hCG or (B) 60 h was recorded. Each time point is the record of least 80 embryos from 3 independent replicates. *p < 0.001, compared to the corresponding stage of cultured embryo. (C) Representative images of embryos stained with Hoechst 33342 and examples of fragmented nuclei shown with arrows.Back to article page