Average weight and blood glucose level in high-fat-diet (HFD)-induced mouse models. (A) At age of 21d (day), the female mice were randomly divided into two groups and fed with CD (control diet) and HFD (high-fat-diet), respectively. Twelve weeks later, mice fed with CD/HFD were mated with normal male mice, respectively. The pups of them were fed with diets as pre-pregnancy until weaning. Weaned male pups were fed with CD. At age of 7–8 weeks, spermatozoa of male pups were collected. After 12 weeks of treatment, the average weight of the HFD (n = 17) group was clearly heavier than that of the CD (n = 13) group, P < 0.001 (B), but the average blood glucose level of CD (n = 13) and HFD (n = 15) was similar between the two groups (C), P = 0.8209.