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Figure 8 | Reproductive Biology and Endocrinology

Figure 8

From: Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process

Figure 8

Testosterone secretion was influenced by the expression of SOCS3 and pSTAT3 level. (A) The expression of SOCS3 and STAT3/pSTAT3 after stimulation of 100 nM leptin was detected by western blotting. (B) The expression of SOCS3 after stimulation of 100 nM leptin could regulate the secretion of testosterone. (C) It was confirmed that AG490 (20 μM) could block STAT3 phosphorylation. (D) The induced role of low concentrations of leptin and inhibited role of high concentrations of leptin both dropped compared with the DMSO-alone group. The means were compared using a one-way analysis of variance followed by Student-Newman-Keuls test to conduct multiple comparisons. *, p < 0.05; NSD, non-significant difference. The photograph is representative of three independent experiments. Each column and bar represents the mean ± SD of three independent experiments.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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