Figure 4

Change in testosterone and offspring was recovered partly by decreasing leptin concentration by the withdrawal of MSG treatment. Mice were treated with MSG from d0 to d14 or d28, and then injected with NS instead of MSG to d56; these groups are called 14MSG-d56 and 28MSG-d56, respectively. The leptin level of the mouse plasma was measured by ELISA (A). Testosterone level of plasma was detected by EIA (B). The methods to measure the testicular weight and volume (C) were described in the Materials and Methods. The total number of offspring was divided by the number of mated female mice to obtain the average number of offspring for each female mouse (D). The means were compared using a one-way analysis of variance followed by Student-Newman-Keuls test to conduct multiple comparisons. *, p < 0.05 compared with the 56MSG-treated mice group; #, p < 0.05 compared with the 56NS-treated mice group; NSD, non-significant difference.