VEGF-D overexpression potentiates lymphatic metastasis of ovarian cancer cell. (A-D) Histologic examination of lymph nodes in the renal hilum reveals the presence of metastasis (indicated by arrows). (A) and (B) are different fields of view of the same section, and so are (C) and (D). (E-G) Lymph nodes are immunostained for CA125 (E and F) and CD40 (G). (H-I) Percentage of lymph nodes with metastasis in the sentinel or total lymph nodes is shown. *P < 0.05 vs. xenografts bearing SKOV3 or SKOV3/pcDNA cells in the sentinel nodes. *P < 0.01 vs. xenografts bearing SKOV3 or SKOV3/pcDNA cells in total lymph nodes. Original magnification: ×400; scale bar = 200 μm. LN, lymph nodes; Met, metastasis.