Figure 3

Flow cytometry results for DNA fragmentation. DNA damage was measured by flow cytometry, FITC-deoxyuridine triphosphate (dUTP) substrate was added to the TdT enzyme binding with the free 3′-OH termini of the single – and double strand DNA. DNA damage was measured by gating the population of cells as negative or positive. PI stains total DNA and FITC-dUTP stains apoptotic cells. A: Representative histogram for a sample that is negative for DNA damage and B: Representative histogram for a sample tested positive for DNA damage by flow cytometry.