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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial

Figure 3

Schematics of the DNA damage by the TUNEL assay. (1) Labeling of DNA strand breaks with TdT, which catalyzes the polymerization of labeled nucleotides to free 3′-OH DNA ends in a template-independent manner (TUNEL reaction) and (2) Fluorescein isothiocynate (FITC)-dUTP is incorporated into nucleotide polymers, and it can be directly detected and quantified by flow cytometry.

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