Figure 4

Effect of increasing D-luciferin (substrate) doses on luciferase activity in intact antral follicles. A total number of 96 follicles were transfected with 3 μg of pGL4 and randomly distributed to different D-luciferin substrate groups (5 μg, 15 μg, 75 μg, 100 μg, 150 μg, and 300 μg groups). After 20 hours of transfection, increasing doses of 5 μg, 15 μg, 75 μg, 100 μg, 150 μg, and 300 μg D-luciferin were injected (10 μl volume) to each follicle. The bioluminescence signal intensity was reported as mean ± SEM of luciferase activity (photon flux; photons/sec). Different letters in a group represent the luciferase activities that are significantly different (P < 0.05).