Figure 1

Porcine ovaries, dissected antral follicles and DNA lipid complex microinjection of ovarian antral follicles. A pair of porcine ovaries in follicular phase of the estrous cycle (A); dissected porcine antral follicles between 6.5 to 7.5 mm in diameter (B); DNA lipid complex microinjection into an antral follicle size between 6.5 to 7.5 mm in diameter on 100 × 15 mm sterile polystyrene disposable petri dish containing approximately 20 ml of CO₂ Independent Medium using the Eppendorf FemtoJet® microinjector under a standard dissecting microscope (C); and representative in vitro antral follicle culture condition on the membrane of Millicell-CM culture plate insert in the 6-well plate with Opti-MEM®-I reduced serum medium (D). Scale bars (A) and (D) = 1.0 cm.