RT-PCR and qRT-PCR detection of AQP8 and AQPs family expression. (A), mRNA expression of AQP family in 4-week-old mouse granulosa cells detected by RT-PCR. (B), mRNA expression of AQP8 in mouse neonatal ovaries detected by RT-PCR. (C), relative mRNA expression levels of AQP5, AQP7, AQP11 and AQP12 in mouse neonatal ovaries detected by qRT-PCR and analyzed by ΔΔCt method. n = 3 separate experiments. *: P < 0.05; ***: P < 0.001 compared with wild-type.