Table 2 Proteins identified in LC-MS/MS from ROS- and ROS+ seminal plasma
From: Proteomic analysis of seminal fluid from men exhibiting oxidative stress
No. | Protein name | NCBI database index number | Calaculated MW (kDa) | pI | ROS- | ROS+ | ||
|---|---|---|---|---|---|---|---|---|
Peptides | Mascot | Peptides | Mascot | |||||
| Â | Â | Â | Â | Â | (Coverage) | Â | (Coverage) | Â |
1 | semenogelin II precursor | 4506885 | 65 | 9.0 | 9 (20%) | 1968 | 7 (10%) | 6998 |
2 | semenogelin I isoform b preproprotein | 38049014 | 45 | 9.2 | 6 (14%) | 1597 | 3 (14%) | 345 |
3 | fibronectin 1 isoform 3 preproprotein | 16933542 | 262 | 5.4 | 5 (3%) | 964 | - | - |
4 | prostate specific antigen isoform 1 preproprotein | 4502173 | 29 | 7.6 | 6 (38%) | 486 | 3 (15%) | 255 |
5 | clusterin isoform 1 | 42716297 | 58 | 6.2 | 2 (3%) | 233 | 4 (12%) | 203 |
6 | macrophage migration inhibitory factor | 4505185 | 12 | 7.7 | 2 (9%) | 206 | - | - |
7 | prolactin-inducible protein precursor | 4505821 | 16 | 8.2 | 3 (48%) | 123 | 5 (44%) | 11771 |
8 | galectin 3 binding protein | 5031863 | 66 | 5.1 | 1 (3%) | 104 | - | - |
9 | albumin preproprotein | 4502027 | 71 | 5.9 | - | - | 7 (17%) | 1092 |
10 | cystatin S precursor | 4503109 | 16 | 4.9 | - | - | 2 (32%) | 188 |
11 | prostate specific antigen isoform 4 preproprotein | 71834855 | 24 | 7.0 | - | - | 2 (10%) | 163 |
12 | zinc alpha-2-glycoprotein 1 | 4502337 | 34 | 5.7 | 1 (7%) | 125 | 2 (12%) | 143 |
13 | lactotransferrin precursor | 54607120 | 80 | 8.5 | - | - | 1 (3%) | 140 |
14 | acid phosphatase, prostate short isoform precursor | 6382064 | 45 | 5.8 | 3 (8%) | 220 | 3 (8%) | 316 |