Table 1 qRT-PCR and BS primers
From: Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst
| Â | Gene | Primers | Ref. |
|---|---|---|---|
qRT-PCR | Dnmt1o | F: 5′-GGTTGATTGAGGGTCATT-3′ | [34] |
R: 5′-GCAGGAATTCATGCAGTAAG-3′ | |||
|  | Dnmt3a | F: 5′-GCCGAATTGTGTCTTGGTGGATGACA-3′ | [36] |
R: 5′-CCTGGTGGAATGCACTGCAGAAGGA-3′ | |||
|  | Dnmt3b | F: 5′-TTCAGTGACCAGTCCTCAGACACGAA-3′ | [36] |
R: 5′-TCAGAAGGCTGGAGACCTCCCTCTT-3′ | |||
|  | Dnmt3L | F: 5′-GTGCGGGTACTGAGCCTTTTTAGA-3′ | [36] |
R: 5′-CGACATTTGTGACATCTTCCACGTA-3′ | |||
|  | Atp5b | F: 5′-GGCCAAGATGTCCTGCTGTT-3′ | [30] |
R: 5′-GCTGGTAGCCTACAGCAGAAGG-3′ | |||
|  | Gapdh | F: 5′-GCCGGGGCTGGCATTGCT-3′ |  |
R: 5′-TTGCTCAGTGTCCTTGCTGGGG-3′ | |||
BS | IAP LRT | F1: 5′-TTGATAGTTGTGTTTTAAGTGGTAAATAAA-3′ | [9] |
R1: 5′-AAAACACCACAAACCAAAATCTTCTAC-3′ | |||
|  |  | F2: 5′-TTGTGTTTTAAGTGGTAAATAAATAATTTG-3′ |  |
R2: 5′-CAAAAAAAACACACAAACCAAAAT-3′ | |||
|  | Line1 5′ | F1: 5′-GTTAGAGAATTTGATAGTTTTTGGAATAGG-3′ | [9] |
R1: 5′-CCAAAACAAAACCTTTCTCAAACACTATAT-3′ | |||
|  |  | F2: 5′-TAGGAAATTAGTTTGAATAGGTGAGAGGT-3′ |  |
R2: 5′-TCAAACACTATATTACTTTAACAATTCCCA-3′ |