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Table 1 qRT-PCR and BS primers

From: Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst

  Gene Primers Ref.
qRT-PCR Dnmt1o F: 5′-GGTTGATTGAGGGTCATT-3′ [34]
R: 5′-GCAGGAATTCATGCAGTAAG-3′
  Dnmt3a F: 5′-GCCGAATTGTGTCTTGGTGGATGACA-3′ [36]
R: 5′-CCTGGTGGAATGCACTGCAGAAGGA-3′
  Dnmt3b F: 5′-TTCAGTGACCAGTCCTCAGACACGAA-3′ [36]
R: 5′-TCAGAAGGCTGGAGACCTCCCTCTT-3′
  Dnmt3L F: 5′-GTGCGGGTACTGAGCCTTTTTAGA-3′ [36]
R: 5′-CGACATTTGTGACATCTTCCACGTA-3′
  Atp5b F: 5′-GGCCAAGATGTCCTGCTGTT-3′ [30]
R: 5′-GCTGGTAGCCTACAGCAGAAGG-3′
  Gapdh F: 5′-GCCGGGGCTGGCATTGCT-3′  
R: 5′-TTGCTCAGTGTCCTTGCTGGGG-3′
BS IAP LRT F1: 5′-TTGATAGTTGTGTTTTAAGTGGTAAATAAA-3′ [9]
R1: 5′-AAAACACCACAAACCAAAATCTTCTAC-3′
   F2: 5′-TTGTGTTTTAAGTGGTAAATAAATAATTTG-3′  
R2: 5′-CAAAAAAAACACACAAACCAAAAT-3′
  Line1 5′ F1: 5′-GTTAGAGAATTTGATAGTTTTTGGAATAGG-3′ [9]
R1: 5′-CCAAAACAAAACCTTTCTCAAACACTATAT-3′
   F2: 5′-TAGGAAATTAGTTTGAATAGGTGAGAGGT-3′  
R2: 5′-TCAAACACTATATTACTTTAACAATTCCCA-3′