mRNA profiles of each PBL fraction. (A) Profiles of ISG15 (a to d), MX1 (e to h), MX2 (i to l), and OAS1 (m to p) mRNA levels in the four PBL fractions: all blood cells except dead cells (all), granulocytes (G), monocytes (M), and lymphocytes (L) in early pregnant (closed circles; n = 4) and cyclic (open circles; n = 3) cows. Values were adjusted for GAPDH mRNA expression. They represent the relative fold change compared with the value on day 0. The error bars represent the SEM. Values significantly different from the value on day 0 of gestation are shown with an asterisk. P values less than 0.05 were considered significant. (B) ISG15 (a), MX1 (b), MX2 (c), and OAS1 (d) mRNA levels in the PBL fractions on day 14 of pregnancy (n = 5). A: all fractions; G: G fraction; M: M fraction; L: L fraction. Values represent the mean values adjusted for GAPDH mRNA expression, and the error bars represent the SEM. Values significantly different from the value in all fractions are shown with an asterisk (p < 0.05).