Figure 3

Immunostaining of the implantation site in the fallopian tube in tubal pregnancy. Representative images showing immunoreactivity of estrogen receptor (ER)-alpha (A, B), progestogen receptor (PR) (C, D), leukemia inhibitory factor (LIF) (E, F), vascular endothelial growth factor (VEGF) (G, H), inducible nitric oxide synthase (iNOS) (I, J), and endocannabinoid receptor (CB1) (K, L). Positive immunolabeling was detected in the epithelial and stromal cells from both the LNG-EC group (A, C, E, G, I) and the control group (B, D, F, H, J). The immunostaining for ER-alpha (A, B) and PR (C, D) was observed in the nuclei of the epithelial and stromal cells from both groups. The positive staining for LIF (E, F), VEGF (G, H), iNOS (I, J), and CB1 (K, L) was found in the cytoplasm of the epithelial and stromal cells from both groups. No significant differences were observed in any of the immunoreactivity of ER-alpha (A, B), PR (C, D), LIF (E, F), VEGF (G, H), iNOS (I, J), or CB1 (K, L) between the two groups. Magnification: 400×.