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Table 1 Identification of proteins in the 12 replicates from NN, NA, ON and OA group showing protein name, NCBI database index, molecular weight, peptide coverage and Mascot score

From: Functional proteomic analysis of seminal plasma proteins in men with various semen parameters

Identification

LTQ

No. Protein name

NCBI database index number

Calculated MW in kDa

PI

Peptides (%coverage)

Mascot score

Sample NN1

semenogelin II precursor

4506885

65

9

8(10%)

9536

prolactin induced protein

4505821

16

8.2

3(20%)

5335

albumin preproprotein

4502027

71

5.9

9(22%)

4805

epididymal secretory protein E1 precursor

5453678

16

7.5

3 (21%)

794

prosaposin isoform a preproprotein

11386147

59

5

1 (2%)

658

mucin 6, gastric

151301154

263

7.2

4 (3%)

461

prostate specific antigen isoform 4 preproprotein

71834855

24

7

3 (15%)

460

armadillo repeat protein

4502247

105

6.3

3(5%)

413

cathepsin D preproprotein

4503143

45

6.1

1 (4%)

285

zinc alpha-2-glycoprotein 1

4502337

34

5.7

2 (12%)

260

cystatin S precursor

4503109

16

4.9

2 (31%)

178

ubiquitin and ribosomal protein S27a precursor

4506713

18

9.6

1 (10%)

173

clusterin isoform 1

42716297

58

6.2

3 (10%)

142

Sample NN2

prolactin-induced protein

4505821

16

8.2

10 (69%)

32886

semenogelin II precursor

4506885

65

9

17 (33%)

22468

semenogelin I isoform b preproprotein

38049014

45

9.2

13 (33%)

5388

albumin preproprotein

4502027

71

5.9

24 (54%)

15508

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

11 (61%)

5087

lactotransferrin precursor

54607120

80

8.5

22 (42%)

4281

zinc alpha-2-glycoprotein 1

4502337

34

5.7

11 (44%)

3424

cystatin S precursor

4503109

16

4.9

5 (44%)

1809

prosaposin isoform a preproprotein

11386147

59

5

9 (33%)

880

epididymal secretory protein E1 precursor

5453678

16

7.5

4 (52%)

786

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

11 (48%)

737

mucin 6, gastric

151301154

263

7.2

11 (9%)

524

extracellular matrix protein 1 isoform 1 precursor

221316614

62

6.2

7 (31%)

504

cystatin C precursor

4503107

16

9

6 (68%)

432

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

4 (40%)

411

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

8(11%)

353

cathepsin D preproprotein

4503143

45

6.1

3 (11%)

266

acid phosphatase, prostate short isoform precursor

6382064

44

5.8

2 (12%)

166

carboxypeptidase E preproprotein

4503009

53

5

5 (20%)

153

clusterin isoform 1

42716297

58

6.2

2 (7%)

106

Sample NN3

prolactin-induced protein

4505821

16

8.2

10 (66%)

24697

semenogelin II precursor

4506885

65

9

19 (35%)

13533

semenogelin I isoform b preproprotein

38049014

45

9.2

15 (39%)

2633

albumin preproprotein

4502027

71

5.9

30 (60%)

8842

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

16 (70%)

8634

lactotransferrin precursor

54607120

80

8.5

23 (49%)

4607

zinc alpha-2-glycoprotein 1

4502337

34

5.7

9 (42%)

3935

epididymal secretory protein E1 precursor

5453678

16

7.5

6 (52%)

1214

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

3 (29%)

987

prosaposin isoform a preproprotein

11386147

59

5

5 (21%)

833

extracellular matrix protein 1 isoform 1 precursor

221316614

62

6.2

6 (22%)

766

cystatin S precursor

4503109

16

4.9

5 (49%)

621

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

523

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

4 (2%)

410

orosomucoid 1 precursor

167857790

23

5

3 (16%)

397

cystatin C precursor

4503107

16

9

3 (26%)

301

mucin 6, gastric

151301154

263

7.2

7 (7%)

288

cathepsin D preproprotein

4503143

45

6.1

2 (11%)

228

carboxypeptidase E preproprotein

4503009

53

5

3 (16%)

193

acidic epididymal glycoprotein-like 1 isoform 1 precursor

25121982

29

5.5

2 (10%)

166

galectin 3 binding protein

5031863

66

5.1

4 (11%)

165

clusterin isoform 1

42716297

58

6.2

2 (7%)

158

acid phosphatase, prostate short isoform precursor

6382064

44

5.8

5 (16%)

128

prostaglandin (H2) D-isomerase −1 peptide

32171249

21

7.6

1 (8%)

115

Sample NN4

prolactin-induced protein

4505821

16

8.2

12 (76%)

36052

semenogelin II precursor

4506885

65

9

16 (32%)

17746

semenogelin I isoform b preproprotein

38049014

45

9.2

14 (34%)

2723

lactotransferrin precursor

54607120

80

8.5

39 (61%)

8695

albumin preproprotein

4502027

71

5.9

23 (48%)

8361

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

15 (67%)

7417

zinc alpha-2-glycoprotein 1

4502337

34

5.7

11 (38%)

3979

mucin 6, gastric

151301154

263

7.2

14 (12%)

1685

epididymal secretory protein E1 precursor

5453678

16

7.5

7 (70%)

1428

acid phosphatase, prostate short isoform precursor

6382064

44

5.8

9 (26%)

1384

clusterin isoform 1

42716297

58

6.2

6 (16%)

1148

orosomucoid 1 precursor

167857790

23

5

4 (32%)

942

orosomucoid 2

4505529

23

5

2 (12%)

207

prosaposin isoform a preproprotein

11386147

59

5

6 (27%)

941

extracellular matrix protein 1 isoform 1 precursor

221316614

62

6.2

7 (25%)

731

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

3 (29%)

635

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

489

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

4 (3%)

447

cystatin C precursor

4503107

16

9

3 (26%)

391

carboxypeptidase E preproprotein

4503009

53

5

4 (14%)

358

galectin 3 binding protein

5031863

66

5.1

5 (14%)

341

transferrin

4557871

79

6.8

2 (4%)

275

acidic epididymal glycoprotein-like 1 isoform 1 precursor

25121982

29

5.5

2 (10%)

238

cystatin S precursor

4503109

16

4.9

2 (20%)

205

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

3 (12%)

187

cathepsin D preproprotein

4503143

45

6.1

2 (11%)

185

prostaglandin (H2) D-isomerase −1 peptide

32171249

21

7.6

1 (8%)

133

Sample NN5

prolactin-induced protein

4505821

16

8.2

8 (44%)

15001

semenogelin II precursor

4506885

65

9

10 (14%)

7235

albumin preproprotein

4502027

71

5.9

17 (39%)

2621

epididymal secretory protein E1 precursor

5453678

16

7.5

3 (23%)

552

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

5 (22%)

539

cystatin S precursor

4503109

16

4.9

2 (23%)

332

zinc alpha-2-glycoprotein 1

4502337

34

5.7

4 (19%)

250

prosaposin isoform a preproprotein

11386147

59

5

1 (2%)

239

prostatic acid phosphatase precursor

6382064

44

5.8

2 (4%)

180

lactotransferrin

54607120

80

8.5

4 (10%)

173

clusterin isoform 1

42716297

58

6.2

2 (7%)

157

galectin 3 binding protein

5031863

66

5.1

2 (4%)

152

extracellular matrix protein 1 isoform 1 precursor

4758236

62

6.2

1 (5%)

119

Sample NA1

prolactin-induced protein

4505821

16

8.2

10 (62%)

17127

albumin preproprotein

4502027

71

5.9

36 (61%)

9157

semenogelin II precursor

4506885

65

9

22 (39%)

7469

semenogelin I isoform b preproprotein

38049014

45

9.2

19 (42%)

2622

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

14 (67%)

5287

lactotransferrin

54607120

80

8.5

19 (40%)

2696

zinc alpha-2-glycoprotein 1

4502337

34

5.7

9 (38%)

1904

epididymal secretory protein E1 precursor

5453678

16

7.5

6 (68%)

1228

extracellular matrix protein 1 isoform 1 precursor

221316614

62

6.2

6 (22%)

635

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

8 (27%)

616

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

2 (19%)

487

prosaposin isoform a preproprotein

11386147

59

5

4 (15%)

475

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

400

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

7 (5%)

398

cystatin C precursor

4503107

16

9

3 (26%)

340

cystatin S precursor

4503109

16

4.9

2 (20%)

261

mucin 6, gastric isoform 1

89033736

185

6.3

4 (5%)

238

prostaglandin (H2) D-isomerase

32171249

21

7.6

1 (8%)

227

prostatic acid phosphatase precursor

6382064

44

5.8

4 (8%)

189

protein tyrosine phosphatase, receptor type, sigma isoform 1 precursor

104487006

218

6.1

5 (5%)

180

transferrin

4557871

79

6.8

3 (17%)

174

clusterin isoform 1

42716297

58

6.2

4 (11%)

171

cathepsin D preproprotein

4503143

45

6.1

1 (4%)

149

galectin 3 binding protein

5031863

66

5.1

5 (14%)

101

Sample NA2

prolactin-induced protein

4505821

16

8.2

12 (76%)

21197

semenogelin II precursor

4506885

65

9

18 (36%)

9137

semenogelin I isoform b preproprotein

38049014

45

9.2

13 (33%)

1777

albumin preproprotein

4502027

71

5.9

26 (55%)

7695

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

16 (67%)

4963

zinc alpha-2-glycoprotein 1

4502337

34

5.7

13 (44%)

3134

lactotransferrin

54607120

80

8.5

24 (46%)

3105

epididymal secretory protein E1 precursor

5453678

16

7.5

5 (68%)

1276

prosaposin isoform a preproprotein

11386147

59

5

7 (26%)

662

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

3 (29%)

660

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

5 (20%)

612

extracellular matrix protein 1 isoform 1 precursor

4758236

62

6.2

7 (22%)

577

cystatin C precursor

4503107

16

9

3 (26%)

548

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

443

prostatic acid phosphatase precursor

6382064

44

5.8

8 (18%)

410

cathepsin D preproprotein

4503143

45

6.1

5 (25%)

375

clusterin isoform 1

42716297

58

6.2

2 (7%)

308

DJ-1 protein

31543380

20

6.3

2 (26%)

279

carboxypeptidase E preproprotein

4503009

53

5

4 (19%)

278

galectin 3 binding protein

5031863

66

5.1

5 (14%)

266

cystatin S precursor

4503109

16

4.9

6 (64%)

212

CD177 molecule

110735433

47

5.6

2 (9%)

199

mucin 6, gastric isoform 1

89033736

185

6.3

3 (4%)

196

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

4 (4%)

172

secretory leukocyte peptidase inhibitor precursor

4507065

15

9.1

2 (28%)

162

cathepsin B preproprotein

4503139

38

5.8

3 (12%)

127

Sample NA3

prolactin-induced protein

4505821

16

8.2

5 (37%)

6642

semenogelin II precursor

4506885

65

9

6 (9%)

5126

albumin preproprotein

4502027

71

5.9

7 (17%)

2077

prosaposin isoform a preproprotein

11386147

59

5

2 (8%)

438

mucin 6, gastric isoform 1

89033736

185

6.3

3 (2%)

269

epididymal secretory protein E1 precursor

5453678

16

7.5

2 (21%)

210

zinc alpha-2-glycoprotein 1

4502337

34

5.7

3 (24%)

203

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

3 (13%)

141

clusterin isoform 1

42716297

58

6.2

3 (10%)

117

Sample NA4

prolactin-induced protein

4505821

16

8.2

10(76%)

22296

semenogelin II precursor

4506885

65

9

16 (30%)

11486

semenogelin I isoform b preproprotein

38049014

45

9.2

13 (33%)

3038

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

14 (67%)

3631

zinc alpha-2-glycoprotein 1

4502337

34

5.7

11 (38%)

3260

lactotransferrin

54607120

80

8.5

16 (45%)

2926

prosaposin isoform a preproprotein

11386147

59

5

5 (20%)

773

epididymal secretory protein E1 precursor

5453678

16

7.5

3 (39%)

533

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

4 (12%)

506

extracellular matrix protein 1 isoform 1 precursor

4758236

62

6.2

4 (17%)

454

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

388

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

3 (29%)

351

cystatin S precursor

4503109

16

4.9

5 (48%)

321

cystatin C precursor

4503107

16

9

2 (26%)

280

cathepsin D preproprotein

4503143

45

6.1

2 (11%)

200

cathepsin B preproprotein

4503139

38

5.8

2 (10%)

186

carboxypeptidase E preproprotein

4503009

53

5

3 (11%)

162

Sample OA1

prolactin-induced protein

4505821

16

8.2

12 (77%)

22670

semenogelin II precursor

4506885

65

9

19(32%)

13764

semenogelin I isoform b preproprotein

38049014

45

9.2

15 (27%)

6586

albumin preproprotein

4502027

71

5.9

26 (51%)

7715

lactotransferrin

54607120

80

8.5

28 (54%)

5063

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

11 (64%)

3309

zinc alpha-2-glycoprotein 1

4502337

34

5.7

9 (40%)

3298

ankyrin repeat domain 11

56676397

299

6.7

3 (1%)

1056

PREDICTED: mucin 6, gastric isoform 1

89033736

185

6.3

10 (14%)

808

epididymal secretory protein E1 precursor

5453678

16

7.5

7 (70%)

635

clusterin isoform 1

42716297

58

6.2

4 (13%)

512

fibronectin 1 isoform 2 preproprotein

47132551

269

5.3

10 (7%)

503

extracellular matrix protein 1 isoform 1 precursor

4758236

62

6.2

5 (28%)

420

prosaposin isoform a preproprotein

11386147

59

5

4 (18%)

407

prostatic acid phosphatase precursor

6382064

44

5.8

9 (28%)

365

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

4 (35%)

337

beta 2 microglobulin precursor – 1 peptide

4757826

13

6

1 (18%)

262

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

4 (12%)

242

transferrin

4557871

79

6.8

3 (4%)

211

cathepsin D preproprotein

4503143

45

6.1

2 (11%)

206

cystatin C precursor

4503107

16

9

5 (52%)

199

cystatin S precursor

4503109

16

4.9

6 (68%)

198

secretory leukocyte peptidase inhibitor precursor −1 peptide

4507065

15

9.1

1 (18%)

175

carboxypeptidase E preproprotein

4503009

53

5

2 (8%)

123

galectin 3 binding protein

5031863

66

5.1

3 (8%)

105

cathepsin B preproprotein

4503139

38

5.8

2 (12%)

95

expressed in prostate and testis

19923082

14

8.2

1 (10%)

86

macrophage migration inhibitory factor – 1 peptide

4505185

12

7.7

1 (9%)

84

prostaglandin (H2) D-isomerase −1 peptide

32171249

21

7.6

1 (8%)

75

Sample OA2

prolactin-induced protein

4505821

16

8.2

8 (51%)

13511

semenogelin II precursor

4506885

65

9

11 (18%)

6235

albumin preproprotein

4502027

71

5.9

10 (23%)

1848

cystatin S precursor

4503109

16

4.9

3 (28%)

522

prostate specific antigen isoform 4 preproprotein

71834855

24

7

4 (25%)

354

prosaposin isoform a preproprotein

11386147

59

5

1 (2%)

324

clusterin isoform 1

42716297

58

6.2

3 (10%)

308

mucin 6, gastric isoform 1

89033736

185

6.3

2 (1%)

306

zinc alpha-2-glycoprotein 1

4502337

34

5.7

4 (17%)

291

epididymal secretory protein E1 precursor

5453678

16

7.5

3 (38%)

184

ankyrin repeat domain 11

56676397

299

6.7

3 (1%)

133

carboxypeptidase E preproprotein

4503009

53

5

2 (8%)

121

lactotransferrin precursor

54607120

80

8.5

3 (9%)

115

extracellular matrix protein 1 isoform 1 precursor

4758236

62

6.2

1 (5%)

108

galectin 3 binding protein

5031863

66

5.1

1 (2%)

98

cathepsin D preproprotein

4503143

45

6.1

2 (13%)

95

lactamase, beta isoform a

26051231

61

8.7

2 (10%)

90

prostatic acid phosphatase precursor

6382064

44

5.8

1 (4%)

78

Sample ON

prolactin-induced protein

4505821

16

8.2

9 (69%)

33212

semenogelin II precursor

4506885

65

9

20 (35%)

10703

semenogelin I isoform b preproprotein

38049014

45

9.2

17 ((38%)

2722

albumin preproprotein

4502027

71

5.9

25 (52%)

9519

prostate specific antigen isoform 1 preproprotein

4502173

29

7.6

14 (62%)

6487

zinc alpha-2-glycoprotein 1

4502337

34

5.7

9 (38%)

5967

lactotransferrin precursor

54607120

80

8.5

18(40%)

3770

serine proteinase inhibitor, clade A, member 1

50363217

46

5.3

4 (15%)

1138

prosaposin isoform a preproprotein

11386147

59

5

6 (26%)

1014

epididymal secretory protein E1 precursor

5453678

16

7.5

3 (39%)

937

tissue inhibitor of metalloproteinase 1 precursor

4507509

23

8.4

2 (19%)

866

extracellular matrix protein 1 isoform 1 precursor

221316614

62

6.2

3 (12%)

637

beta 2 microglobulin precursor

4757826

13

6

2 (21%)

633

mucin 6, gastric

151301154

263

7.2

5 (5%)

407

fibronectin 1 isoform 3 preproprotein

16933542

262

5.4

2 (1%)

352

cathepsin D preproprotein

4503143

45

6.1

2 (11%)

334

cystatin C precursor

4503107

16

9

3 (26%)

320

carboxypeptidase E preproprotein

4503009

53

5

3 (11%)

172

clusterin isoform 1

42716297

58

6.2

2 (7%)

147

acid phosphatase, prostate short isoform precursor

6382064

44

5.8

3 (8%)

132

secretory leukocyte peptidase inhibitor precursor

4507065

15

9.1

2 (42%)

123

protein tyrosine phosphatase, receptor type, sigma isoform 1 precursor

104487006

218

6.1

4 (3%)

112