Skip to main content

Table 1 Identification of proteins in the 12 replicates from NN, NA, ON and OA group showing protein name, NCBI database index, molecular weight, peptide coverage and Mascot score

From: Functional proteomic analysis of seminal plasma proteins in men with various semen parameters

Identification LTQ
No. Protein name NCBI database index number Calculated MW in kDa PI Peptides (%coverage) Mascot score
Sample NN1
semenogelin II precursor 4506885 65 9 8(10%) 9536
prolactin induced protein 4505821 16 8.2 3(20%) 5335
albumin preproprotein 4502027 71 5.9 9(22%) 4805
epididymal secretory protein E1 precursor 5453678 16 7.5 3 (21%) 794
prosaposin isoform a preproprotein 11386147 59 5 1 (2%) 658
mucin 6, gastric 151301154 263 7.2 4 (3%) 461
prostate specific antigen isoform 4 preproprotein 71834855 24 7 3 (15%) 460
armadillo repeat protein 4502247 105 6.3 3(5%) 413
cathepsin D preproprotein 4503143 45 6.1 1 (4%) 285
zinc alpha-2-glycoprotein 1 4502337 34 5.7 2 (12%) 260
cystatin S precursor 4503109 16 4.9 2 (31%) 178
ubiquitin and ribosomal protein S27a precursor 4506713 18 9.6 1 (10%) 173
clusterin isoform 1 42716297 58 6.2 3 (10%) 142
Sample NN2
prolactin-induced protein 4505821 16 8.2 10 (69%) 32886
semenogelin II precursor 4506885 65 9 17 (33%) 22468
semenogelin I isoform b preproprotein 38049014 45 9.2 13 (33%) 5388
albumin preproprotein 4502027 71 5.9 24 (54%) 15508
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 11 (61%) 5087
lactotransferrin precursor 54607120 80 8.5 22 (42%) 4281
zinc alpha-2-glycoprotein 1 4502337 34 5.7 11 (44%) 3424
cystatin S precursor 4503109 16 4.9 5 (44%) 1809
prosaposin isoform a preproprotein 11386147 59 5 9 (33%) 880
epididymal secretory protein E1 precursor 5453678 16 7.5 4 (52%) 786
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 11 (48%) 737
mucin 6, gastric 151301154 263 7.2 11 (9%) 524
extracellular matrix protein 1 isoform 1 precursor 221316614 62 6.2 7 (31%) 504
cystatin C precursor 4503107 16 9 6 (68%) 432
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 4 (40%) 411
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 8(11%) 353
cathepsin D preproprotein 4503143 45 6.1 3 (11%) 266
acid phosphatase, prostate short isoform precursor 6382064 44 5.8 2 (12%) 166
carboxypeptidase E preproprotein 4503009 53 5 5 (20%) 153
clusterin isoform 1 42716297 58 6.2 2 (7%) 106
Sample NN3
prolactin-induced protein 4505821 16 8.2 10 (66%) 24697
semenogelin II precursor 4506885 65 9 19 (35%) 13533
semenogelin I isoform b preproprotein 38049014 45 9.2 15 (39%) 2633
albumin preproprotein 4502027 71 5.9 30 (60%) 8842
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 16 (70%) 8634
lactotransferrin precursor 54607120 80 8.5 23 (49%) 4607
zinc alpha-2-glycoprotein 1 4502337 34 5.7 9 (42%) 3935
epididymal secretory protein E1 precursor 5453678 16 7.5 6 (52%) 1214
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 3 (29%) 987
prosaposin isoform a preproprotein 11386147 59 5 5 (21%) 833
extracellular matrix protein 1 isoform 1 precursor 221316614 62 6.2 6 (22%) 766
cystatin S precursor 4503109 16 4.9 5 (49%) 621
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 523
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 4 (2%) 410
orosomucoid 1 precursor 167857790 23 5 3 (16%) 397
cystatin C precursor 4503107 16 9 3 (26%) 301
mucin 6, gastric 151301154 263 7.2 7 (7%) 288
cathepsin D preproprotein 4503143 45 6.1 2 (11%) 228
carboxypeptidase E preproprotein 4503009 53 5 3 (16%) 193
acidic epididymal glycoprotein-like 1 isoform 1 precursor 25121982 29 5.5 2 (10%) 166
galectin 3 binding protein 5031863 66 5.1 4 (11%) 165
clusterin isoform 1 42716297 58 6.2 2 (7%) 158
acid phosphatase, prostate short isoform precursor 6382064 44 5.8 5 (16%) 128
prostaglandin (H2) D-isomerase −1 peptide 32171249 21 7.6 1 (8%) 115
Sample NN4
prolactin-induced protein 4505821 16 8.2 12 (76%) 36052
semenogelin II precursor 4506885 65 9 16 (32%) 17746
semenogelin I isoform b preproprotein 38049014 45 9.2 14 (34%) 2723
lactotransferrin precursor 54607120 80 8.5 39 (61%) 8695
albumin preproprotein 4502027 71 5.9 23 (48%) 8361
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 15 (67%) 7417
zinc alpha-2-glycoprotein 1 4502337 34 5.7 11 (38%) 3979
mucin 6, gastric 151301154 263 7.2 14 (12%) 1685
epididymal secretory protein E1 precursor 5453678 16 7.5 7 (70%) 1428
acid phosphatase, prostate short isoform precursor 6382064 44 5.8 9 (26%) 1384
clusterin isoform 1 42716297 58 6.2 6 (16%) 1148
orosomucoid 1 precursor 167857790 23 5 4 (32%) 942
orosomucoid 2 4505529 23 5 2 (12%) 207
prosaposin isoform a preproprotein 11386147 59 5 6 (27%) 941
extracellular matrix protein 1 isoform 1 precursor 221316614 62 6.2 7 (25%) 731
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 3 (29%) 635
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 489
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 4 (3%) 447
cystatin C precursor 4503107 16 9 3 (26%) 391
carboxypeptidase E preproprotein 4503009 53 5 4 (14%) 358
galectin 3 binding protein 5031863 66 5.1 5 (14%) 341
transferrin 4557871 79 6.8 2 (4%) 275
acidic epididymal glycoprotein-like 1 isoform 1 precursor 25121982 29 5.5 2 (10%) 238
cystatin S precursor 4503109 16 4.9 2 (20%) 205
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 3 (12%) 187
cathepsin D preproprotein 4503143 45 6.1 2 (11%) 185
prostaglandin (H2) D-isomerase −1 peptide 32171249 21 7.6 1 (8%) 133
Sample NN5
prolactin-induced protein 4505821 16 8.2 8 (44%) 15001
semenogelin II precursor 4506885 65 9 10 (14%) 7235
albumin preproprotein 4502027 71 5.9 17 (39%) 2621
epididymal secretory protein E1 precursor 5453678 16 7.5 3 (23%) 552
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 5 (22%) 539
cystatin S precursor 4503109 16 4.9 2 (23%) 332
zinc alpha-2-glycoprotein 1 4502337 34 5.7 4 (19%) 250
prosaposin isoform a preproprotein 11386147 59 5 1 (2%) 239
prostatic acid phosphatase precursor 6382064 44 5.8 2 (4%) 180
lactotransferrin 54607120 80 8.5 4 (10%) 173
clusterin isoform 1 42716297 58 6.2 2 (7%) 157
galectin 3 binding protein 5031863 66 5.1 2 (4%) 152
extracellular matrix protein 1 isoform 1 precursor 4758236 62 6.2 1 (5%) 119
Sample NA1
prolactin-induced protein 4505821 16 8.2 10 (62%) 17127
albumin preproprotein 4502027 71 5.9 36 (61%) 9157
semenogelin II precursor 4506885 65 9 22 (39%) 7469
semenogelin I isoform b preproprotein 38049014 45 9.2 19 (42%) 2622
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 14 (67%) 5287
lactotransferrin 54607120 80 8.5 19 (40%) 2696
zinc alpha-2-glycoprotein 1 4502337 34 5.7 9 (38%) 1904
epididymal secretory protein E1 precursor 5453678 16 7.5 6 (68%) 1228
extracellular matrix protein 1 isoform 1 precursor 221316614 62 6.2 6 (22%) 635
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 8 (27%) 616
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 2 (19%) 487
prosaposin isoform a preproprotein 11386147 59 5 4 (15%) 475
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 400
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 7 (5%) 398
cystatin C precursor 4503107 16 9 3 (26%) 340
cystatin S precursor 4503109 16 4.9 2 (20%) 261
mucin 6, gastric isoform 1 89033736 185 6.3 4 (5%) 238
prostaglandin (H2) D-isomerase 32171249 21 7.6 1 (8%) 227
prostatic acid phosphatase precursor 6382064 44 5.8 4 (8%) 189
protein tyrosine phosphatase, receptor type, sigma isoform 1 precursor 104487006 218 6.1 5 (5%) 180
transferrin 4557871 79 6.8 3 (17%) 174
clusterin isoform 1 42716297 58 6.2 4 (11%) 171
cathepsin D preproprotein 4503143 45 6.1 1 (4%) 149
galectin 3 binding protein 5031863 66 5.1 5 (14%) 101
Sample NA2
prolactin-induced protein 4505821 16 8.2 12 (76%) 21197
semenogelin II precursor 4506885 65 9 18 (36%) 9137
semenogelin I isoform b preproprotein 38049014 45 9.2 13 (33%) 1777
albumin preproprotein 4502027 71 5.9 26 (55%) 7695
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 16 (67%) 4963
zinc alpha-2-glycoprotein 1 4502337 34 5.7 13 (44%) 3134
lactotransferrin 54607120 80 8.5 24 (46%) 3105
epididymal secretory protein E1 precursor 5453678 16 7.5 5 (68%) 1276
prosaposin isoform a preproprotein 11386147 59 5 7 (26%) 662
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 3 (29%) 660
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 5 (20%) 612
extracellular matrix protein 1 isoform 1 precursor 4758236 62 6.2 7 (22%) 577
cystatin C precursor 4503107 16 9 3 (26%) 548
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 443
prostatic acid phosphatase precursor 6382064 44 5.8 8 (18%) 410
cathepsin D preproprotein 4503143 45 6.1 5 (25%) 375
clusterin isoform 1 42716297 58 6.2 2 (7%) 308
DJ-1 protein 31543380 20 6.3 2 (26%) 279
carboxypeptidase E preproprotein 4503009 53 5 4 (19%) 278
galectin 3 binding protein 5031863 66 5.1 5 (14%) 266
cystatin S precursor 4503109 16 4.9 6 (64%) 212
CD177 molecule 110735433 47 5.6 2 (9%) 199
mucin 6, gastric isoform 1 89033736 185 6.3 3 (4%) 196
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 4 (4%) 172
secretory leukocyte peptidase inhibitor precursor 4507065 15 9.1 2 (28%) 162
cathepsin B preproprotein 4503139 38 5.8 3 (12%) 127
Sample NA3
prolactin-induced protein 4505821 16 8.2 5 (37%) 6642
semenogelin II precursor 4506885 65 9 6 (9%) 5126
albumin preproprotein 4502027 71 5.9 7 (17%) 2077
prosaposin isoform a preproprotein 11386147 59 5 2 (8%) 438
mucin 6, gastric isoform 1 89033736 185 6.3 3 (2%) 269
epididymal secretory protein E1 precursor 5453678 16 7.5 2 (21%) 210
zinc alpha-2-glycoprotein 1 4502337 34 5.7 3 (24%) 203
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 3 (13%) 141
clusterin isoform 1 42716297 58 6.2 3 (10%) 117
Sample NA4
prolactin-induced protein 4505821 16 8.2 10(76%) 22296
semenogelin II precursor 4506885 65 9 16 (30%) 11486
semenogelin I isoform b preproprotein 38049014 45 9.2 13 (33%) 3038
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 14 (67%) 3631
zinc alpha-2-glycoprotein 1 4502337 34 5.7 11 (38%) 3260
lactotransferrin 54607120 80 8.5 16 (45%) 2926
prosaposin isoform a preproprotein 11386147 59 5 5 (20%) 773
epididymal secretory protein E1 precursor 5453678 16 7.5 3 (39%) 533
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 4 (12%) 506
extracellular matrix protein 1 isoform 1 precursor 4758236 62 6.2 4 (17%) 454
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 388
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 3 (29%) 351
cystatin S precursor 4503109 16 4.9 5 (48%) 321
cystatin C precursor 4503107 16 9 2 (26%) 280
cathepsin D preproprotein 4503143 45 6.1 2 (11%) 200
cathepsin B preproprotein 4503139 38 5.8 2 (10%) 186
carboxypeptidase E preproprotein 4503009 53 5 3 (11%) 162
Sample OA1
prolactin-induced protein 4505821 16 8.2 12 (77%) 22670
semenogelin II precursor 4506885 65 9 19(32%) 13764
semenogelin I isoform b preproprotein 38049014 45 9.2 15 (27%) 6586
albumin preproprotein 4502027 71 5.9 26 (51%) 7715
lactotransferrin 54607120 80 8.5 28 (54%) 5063
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 11 (64%) 3309
zinc alpha-2-glycoprotein 1 4502337 34 5.7 9 (40%) 3298
ankyrin repeat domain 11 56676397 299 6.7 3 (1%) 1056
PREDICTED: mucin 6, gastric isoform 1 89033736 185 6.3 10 (14%) 808
epididymal secretory protein E1 precursor 5453678 16 7.5 7 (70%) 635
clusterin isoform 1 42716297 58 6.2 4 (13%) 512
fibronectin 1 isoform 2 preproprotein 47132551 269 5.3 10 (7%) 503
extracellular matrix protein 1 isoform 1 precursor 4758236 62 6.2 5 (28%) 420
prosaposin isoform a preproprotein 11386147 59 5 4 (18%) 407
prostatic acid phosphatase precursor 6382064 44 5.8 9 (28%) 365
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 4 (35%) 337
beta 2 microglobulin precursor – 1 peptide 4757826 13 6 1 (18%) 262
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 4 (12%) 242
transferrin 4557871 79 6.8 3 (4%) 211
cathepsin D preproprotein 4503143 45 6.1 2 (11%) 206
cystatin C precursor 4503107 16 9 5 (52%) 199
cystatin S precursor 4503109 16 4.9 6 (68%) 198
secretory leukocyte peptidase inhibitor precursor −1 peptide 4507065 15 9.1 1 (18%) 175
carboxypeptidase E preproprotein 4503009 53 5 2 (8%) 123
galectin 3 binding protein 5031863 66 5.1 3 (8%) 105
cathepsin B preproprotein 4503139 38 5.8 2 (12%) 95
expressed in prostate and testis 19923082 14 8.2 1 (10%) 86
macrophage migration inhibitory factor – 1 peptide 4505185 12 7.7 1 (9%) 84
prostaglandin (H2) D-isomerase −1 peptide 32171249 21 7.6 1 (8%) 75
Sample OA2
prolactin-induced protein 4505821 16 8.2 8 (51%) 13511
semenogelin II precursor 4506885 65 9 11 (18%) 6235
albumin preproprotein 4502027 71 5.9 10 (23%) 1848
cystatin S precursor 4503109 16 4.9 3 (28%) 522
prostate specific antigen isoform 4 preproprotein 71834855 24 7 4 (25%) 354
prosaposin isoform a preproprotein 11386147 59 5 1 (2%) 324
clusterin isoform 1 42716297 58 6.2 3 (10%) 308
mucin 6, gastric isoform 1 89033736 185 6.3 2 (1%) 306
zinc alpha-2-glycoprotein 1 4502337 34 5.7 4 (17%) 291
epididymal secretory protein E1 precursor 5453678 16 7.5 3 (38%) 184
ankyrin repeat domain 11 56676397 299 6.7 3 (1%) 133
carboxypeptidase E preproprotein 4503009 53 5 2 (8%) 121
lactotransferrin precursor 54607120 80 8.5 3 (9%) 115
extracellular matrix protein 1 isoform 1 precursor 4758236 62 6.2 1 (5%) 108
galectin 3 binding protein 5031863 66 5.1 1 (2%) 98
cathepsin D preproprotein 4503143 45 6.1 2 (13%) 95
lactamase, beta isoform a 26051231 61 8.7 2 (10%) 90
prostatic acid phosphatase precursor 6382064 44 5.8 1 (4%) 78
Sample ON
prolactin-induced protein 4505821 16 8.2 9 (69%) 33212
semenogelin II precursor 4506885 65 9 20 (35%) 10703
semenogelin I isoform b preproprotein 38049014 45 9.2 17 ((38%) 2722
albumin preproprotein 4502027 71 5.9 25 (52%) 9519
prostate specific antigen isoform 1 preproprotein 4502173 29 7.6 14 (62%) 6487
zinc alpha-2-glycoprotein 1 4502337 34 5.7 9 (38%) 5967
lactotransferrin precursor 54607120 80 8.5 18(40%) 3770
serine proteinase inhibitor, clade A, member 1 50363217 46 5.3 4 (15%) 1138
prosaposin isoform a preproprotein 11386147 59 5 6 (26%) 1014
epididymal secretory protein E1 precursor 5453678 16 7.5 3 (39%) 937
tissue inhibitor of metalloproteinase 1 precursor 4507509 23 8.4 2 (19%) 866
extracellular matrix protein 1 isoform 1 precursor 221316614 62 6.2 3 (12%) 637
beta 2 microglobulin precursor 4757826 13 6 2 (21%) 633
mucin 6, gastric 151301154 263 7.2 5 (5%) 407
fibronectin 1 isoform 3 preproprotein 16933542 262 5.4 2 (1%) 352
cathepsin D preproprotein 4503143 45 6.1 2 (11%) 334
cystatin C precursor 4503107 16 9 3 (26%) 320
carboxypeptidase E preproprotein 4503009 53 5 3 (11%) 172
clusterin isoform 1 42716297 58 6.2 2 (7%) 147
acid phosphatase, prostate short isoform precursor 6382064 44 5.8 3 (8%) 132
secretory leukocyte peptidase inhibitor precursor 4507065 15 9.1 2 (42%) 123
protein tyrosine phosphatase, receptor type, sigma isoform 1 precursor 104487006 218 6.1 4 (3%) 112