Figure 1

Comparison of follicle growth in response to FSH treatment in WT and AHRKO follicles. Early antral follicles from pre-pubertal WT (A) and AHRKO (B) ovaries were cultured in supplemented media in the presence of 0–15 IU/mL FSH for 7 days. Growth of follicles was monitored every 24 h by measuring follicle diameter in perpendicular axes and plotted as follicle diameters. (C) Total percent change in WT and AHRKO follicle diameters from day 0 to day 7 of culture. Graphs represent the mean ± SEM from three separate cultures, each with 10–16 follicles per genotype. The asterisk (*) above the dotted line indicates significant differences (p ≤ 0.05) from 0 IU/mL FSH at the same day of follicle growth, using a GLM followed by Tukey’s test. The letter “a” above the bars indicates significant difference (p ≤ 0.05) from WT follicles within the same FSH treatment group and the letter “b” above the bar indicates significant difference (p ≤ 0.05) from 0 IU/mL FSH in the same genotype, using one-way ANOVA followed by Tukey’s test as a post hoc test.