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Table 2 Effect of anti-Prt serum on embryo and cleavage rates

From: Inhibition of ovine in vitro fertilization by anti-Prt antibody: hypothetical model for Prt/ZP interaction

Treatment

Inseminated

oocytes (n)

Cleavage n (%)

D6/7 embryosn (%)

Control

127

(63) 44.1 ± 4.15a

(9) 21.0 ± 5.12

APPA

118

(27) 19.7 ± 4.22b

(6) 24.3 ± 7.12

CSerum

129

(47) 32.2 ± 4.06ab

(8) 22.9 ± 5.56

  1. Effect of spermatozoa incubation in the presence of anti-Prt serum (APPA) or pre-immune serum (CSerum) in the fertilization medium on embryo production rates in 4 replicates for cleavage and 3 replicates for D6/7 embryos (least squares means ± standard error). n: total number; data within columns with different superscripts are statistically different (P ≤ 0.05); Cleavage rate: number of cleaved embryos per number of inseminated oocytes; D6-7 embryo rate as the number of morulae and blastocyts at these days per number of cleaved embryos.