Treatment | Inseminated oocytes (n) | Cleavage n (%) | D6/7 embryosn (%) |
---|
Control | 127 | (63) 44.1 ± 4.15a | (9) 21.0 ± 5.12 |
APPA | 118 | (27) 19.7 ± 4.22b | (6) 24.3 ± 7.12 |
CSerum | 129 | (47) 32.2 ± 4.06ab | (8) 22.9 ± 5.56 |
- Effect of spermatozoa incubation in the presence of anti-Prt serum (APPA) or pre-immune serum (CSerum) in the fertilization medium on embryo production rates in 4 replicates for cleavage and 3 replicates for D6/7 embryos (least squares means ± standard error). n: total number; data within columns with different superscripts are statistically different (P ≤ 0.05); Cleavage rate: number of cleaved embryos per number of inseminated oocytes; D6-7 embryo rate as the number of morulae and blastocyts at these days per number of cleaved embryos.