|
Treatment
|
MII
|
Decond
|
2 pronuclei
|
Synkaryosis
|
2-4 cells
|
Polyspermy
|
Fertilization
|
|---|
| |
(n) %
|
(n) %
|
(n) %
|
(n) %
|
(n) %
|
(n) %
|
(n) %
|
|---|
|
Control
|
(10) 21.5 ± 7.47a
|
(1) 1.9 ± 2.76
|
(20) 52.5 ± 7.78
|
(1) 2.4 ± 3.31 ab
|
(4) 11.0 ± 4.1
|
(3) 9.6 ± 3.45
|
(29) 78.5 ± 7.47a
|
|
APPA
|
(27) 54.0 ± 6.79b
|
(1) 1.6 ± 2.51
|
(13) 29.5 ± 7.07
|
(0) 0.0 ± 3.01b
|
(3) 7.0 ± 3.7
|
(3) 8.0 ± 3.14
|
(20) 46.0 ± 6.79b
|
|
CSerum
|
(19) 35.5 ± 6.45a
|
(2) 3.5 ± 2.46
|
(20) 42.6 ± 6.92
|
(5) 10.2 ± 3.00a
|
(2) 4.7 ± 3.6
|
(1) 3.6 ± 3.07
|
(30) 64.5 ± 6.45a
|
- Assessment of in vitro fertilization of ovine oocytes incubated in the absence (Control) and presence of anti-Prt serum (APPA) and pre-immune serum (Cserum) in 4 replicates. MII: metaphase II; Decond: sperm head decondensation; 2–4 cells: 2–4 cell embryos; Least squares mean values within columns with different letters differ statistically (P ≤ 0.05).
