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Table 1 Assessment of in vitro fertilization of ovine oocytes

From: Inhibition of ovine in vitro fertilization by anti-Prt antibody: hypothetical model for Prt/ZP interaction

Treatment MII Decond 2 pronuclei Synkaryosis 2-4 cells Polyspermy Fertilization
  (n) % (n) % (n) % (n) % (n) % (n) % (n) %
Control (10) 21.5 ± 7.47a (1) 1.9 ± 2.76 (20) 52.5 ± 7.78 (1) 2.4 ± 3.31 ab (4) 11.0 ± 4.1 (3) 9.6 ± 3.45 (29) 78.5 ± 7.47a
APPA (27) 54.0 ± 6.79b (1) 1.6 ± 2.51 (13) 29.5 ± 7.07 (0) 0.0 ± 3.01b (3) 7.0 ± 3.7 (3) 8.0 ± 3.14 (20) 46.0 ± 6.79b
CSerum (19) 35.5 ± 6.45a (2) 3.5 ± 2.46 (20) 42.6 ± 6.92 (5) 10.2 ± 3.00a (2) 4.7 ± 3.6 (1) 3.6 ± 3.07 (30) 64.5 ± 6.45a
  1. Assessment of in vitro fertilization of ovine oocytes incubated in the absence (Control) and presence of anti-Prt serum (APPA) and pre-immune serum (Cserum) in 4 replicates. MII: metaphase II; Decond: sperm head decondensation; 2–4 cells: 2–4 cell embryos; Least squares mean values within columns with different letters differ statistically (P ≤ 0.05).