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Table 1 Assessment of in vitro fertilization of ovine oocytes

From: Inhibition of ovine in vitro fertilization by anti-Prt antibody: hypothetical model for Prt/ZP interaction

Treatment

MII

Decond

2 pronuclei

Synkaryosis

2-4 cells

Polyspermy

Fertilization

 

(n) %

(n) %

(n) %

(n) %

(n) %

(n) %

(n) %

Control

(10) 21.5 ± 7.47a

(1) 1.9 ± 2.76

(20) 52.5 ± 7.78

(1) 2.4 ± 3.31 ab

(4) 11.0 ± 4.1

(3) 9.6 ± 3.45

(29) 78.5 ± 7.47a

APPA

(27) 54.0 ± 6.79b

(1) 1.6 ± 2.51

(13) 29.5 ± 7.07

(0) 0.0 ± 3.01b

(3) 7.0 ± 3.7

(3) 8.0 ± 3.14

(20) 46.0 ± 6.79b

CSerum

(19) 35.5 ± 6.45a

(2) 3.5 ± 2.46

(20) 42.6 ± 6.92

(5) 10.2 ± 3.00a

(2) 4.7 ± 3.6

(1) 3.6 ± 3.07

(30) 64.5 ± 6.45a

  1. Assessment of in vitro fertilization of ovine oocytes incubated in the absence (Control) and presence of anti-Prt serum (APPA) and pre-immune serum (Cserum) in 4 replicates. MII: metaphase II; Decond: sperm head decondensation; 2–4 cells: 2–4 cell embryos; Least squares mean values within columns with different letters differ statistically (P ≤ 0.05).