Table 1 The primer sequences used for real-time quantitative PCR
From: CREBZF expression and hormonal regulation in the mouse uterus
Target gene | GenBank accession no. | Primer sequence | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
SMILE | NM_145151.2 | AF: 5'-TAATCGGCTCAAGAAGAAGG-3' | 144 | 60 |
AR: 5'-CGTAGGTAGCGACTCTCC-3' | ||||
RPLP0 | NM_007475 | AF:5'- GGACCCGAGAAGACCTCCTT-3' | 85 | 60 |
AR:5'- GCACATCACTCAGAATTTCAATGG-3' | ||||
GAPDH | NM_008084 | AF:5'- TCACTGCCACCCAGAAGA-3' | 186 | 60 |
AR:5'- GACGGACACATTGGGGGTAG-3' |