Table 1 Oligonucleotide primer sequences for LHR, AR, AMH, SOX9, ARA54, ARA70 and β-actin used in real-time quantitative RT-PCR
Gene | Oligo-sequence(5′ → 3′) |
|---|---|
LHR | F: TCA.ATT.CTT.GTG.CCA.ATC.CA |
R: CCA.TTT.TTG.CAG.TTG.GAG.GT | |
AR | F: TCA.CCG.CAC.CTG.ATG.TGT.G |
R: ACA.TGG.TCC.CTG.GCA.GTC.TC | |
AMH | F: AGC.TGT.GGG.CAC.CAG.TGG. |
R: GCT.CTT.GTG.GGC.TGC.CTG. | |
SOX 9 | F: GCA.AAG.GAG.ATG.AAA.TCT.GTT.CTG |
R: AAG.GTT.AAC.TGC.TGG.TGT.TCT.GAG.A | |
ARA54 | F: TCT.GCC.TCC.ACT.TGT.GCT.GA |
R: GCC.ACG.GTG.TTC.TTC.CCA.TA | |
ARA70 | F: TGG.AGC.TTG.CTA.TTG.GTG.GAG |
R: CAG.GTG.ACG.GCT.TAT.GCA.ACT | |
ß-actin 18S | F: TCA.CCC.ACA.CTG.TGC.CCA.TCT.ACG.A |
R: CAG.CGG.AAC.CGC.TCA.TTG.CCA.ATG.G | |
F:GTA.ACC.CGT.TGA.ACC.CCA.TT | |
R:CCA.TCC.AAT.CGG.TAG.TAG.CG |