RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients

Table 2 The primer sequences (5’-3’) used in qRT-PCR

Gene symbol	Accession nr	Forward primer	Reversed primer	Fragment length
GAPDH	NM_002046	TGTTGCCATCAATGACCCCTT	CTCCACGACGTACTCAGCG	202
RUNX2	NM_001015051.3	TCCAGGAGGACAGCAAGAAGT	TGTCACTGTGCTGAAGAGGCT	182
PSAT1	NM_021154 .3	ATGGGCTTGGTTCTGGAGTG	GGCCAGCTTCTGAACGTCTT	324
ADAMTS9	NM_182920.1	AGGGTCGTTTTAGCATCAACC	TCCACAGTAACCACCGCATT	149
CXCL1	NM_001511.2	CCCAAACCGAAGTCATAGCC	CCTTCAGGAACAGCCACCAG	158
CXCL2	NM_002089.3	CCCAAACCGAAGTCATAGCC	CAGGAACAGCCACCAATAAGC	154
CXCL3	NM_002090.2	CACTCAAGAATGGGAAGAAAGC	GCAGGAAGTGTCAATGATACGC	142
ITGB5	NM_002213.3	GACCGGAGGGAGTTTGCAA	CCTCGGAGAAGGAAACATCAGT	170
GPX3	NM_002084.3	GGCTTTGTCCCTAATTTCCAG	AAAGTTCCAGCGGATGTCGT	171
PTX3	NM_002852.3	GCATCTCCTTGCGATTCTGTT	CATTCCGAGTGCTCCTGACC	165

ISSN: 1477-7827