Transcripts levels of the target genes ( RUNX2 , PSAT1 , ADAMTS9 , CXCL1 , CXCL2 , CXCL3 , ITGB5 , GPX3 and PTX3 ) according to the fertilization process in PCOS patients. mRNA expression of candidate genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, ITGB5, GPX3 and PTX3) in cumulus cells of PCOS patients, organized according to pronuclei formation after fertilization (normal two pronuclei versus multi pronuclei). The signal intensity for each gene is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogeneous reference. * indicates a significant difference in gene expression between CC categories (** p < 0.01, * p < 0.05). The results are presented as the means ± SEM. CC2PN: cumulus cells from oocyte which formed two normal pronuclei (2PN) after fertilization; CCMPN, cumulus cells from oocyte which formed multi pronuclei (MPN) after fertilization.