Figure 6

Flow cytometric analysis of Fas expression on the surface of cells of early and late stage bovine CL following K8/K18 filament disruption with acrylamide. Representative histograms depicting the expression of Fas on the surface of bovine luteal cells of early stage and late stage CL are shown (Figure 6A and B, respectively). The relative percentage of cells expressing Fas on the cell surface is depicted for early versus late stage CL, and for control versus acrylamide-treated cells (Figure 6C). Relative mean fluorescence intensity (MFI) is also depicted for the two stages of CL and the treatment conditions (Figure 6D). Values shown are mean ± SEM; different letters indicate significant differences (P<0.05; n=4 CL/stage).