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Table 1 List of primers used for RT-PCR, RACE PCR and Real Time PCR

From: Steroidogenic capacity of the placenta as a supplemental source of progesterone during pregnancy in domestic cats

Primer no. Gene Primer sequences Accession number
1. Initial 3βHSD for RT-PCR FWD 5′-TTGATACCCCGGTTTGACCA −3′ AY739720
2. Initial 3βHSD for RT-PCR REV 5′-TTGGCACCCCTAGATCAGTG −3′  
3. ACTB for RT-PCR FWD 5′-ATCAAGGAGAAGCTGTGCTACGT-3′ AB51104
4. ACTB or RT-PCR REV 5′-CGTTGCCGATGGTGATCA −3′  
5. 3βHSD RACE PCR GSP FWD 5′-CACCAAAGCTATGATAACCTCAAT −3′ AY739720
6. 3βHSD RACE PCR GSP REV 5′-AGGCAAGCCAGTACTCCAGAAAT −3′  
7. Universal Primer Mix (UPM) FWD 5′-CTAATACGACTCACTATAGGGCAAGCAG -
   TGGTATCAACGCAGAGT-3′  
8. UPM REV 5′-CTAATACGACTCACTATAGGGC-3′ AY739720
9. 3βHSD for RT-PCR (ORF) FWD 5′-TTGATACCCCGGTTTGACCA −3′  
10. 3βHSD for RT-PCR (ORF) REV 5′-TTGGCACCCCTAGATCAGTG −3′ JF794032
11. 3βHSD specific for Real Time PCR FWD 5′-TCCCCAGTGTTTCTGATTCC −3′  
12. 3βHSD specific for Real Time PCR REV 5′-CACCAACAAATGCACGATTC −3′ EF522840
13. Initial StAR for RT-PCR FWD 5′-CAATGCTCCTAGCGACGTTC −3′  
14. Initial StAR for RT-PCR REV 5′-AACAGTTGGAAGCAGCAGG −3′  
15. StAR for Real Time PCR FWD 5′-CCCATGGAGAGGCTTTATGA-3′ JF800676
16. StAR for Real Time PCR REV 5′-CAACTCGTGGGTGATGACTG −3′  
17. Cyclophilin for Real Time PCR FWD 5′-CCTTCTGTAGCTCGGGTGAG −3′ AY029366
18. Cyclophilin For Real Time PCR REV 5′-CTTGGAGGGGAGGTAAGGAG −3′