Figure 2

Cell viability test and SOD1 mRNA expression in LECs. Dose-dependent effects of H₂O₂ on the cell viability and dose-dependent effects of PGF and H₂O₂ on SOD1 mRNA expression in cultured bovine LECs. The cells were cultured with H₂O₂ (1, 10, 100 and 1000 μM) (Figure2A) for 24 h for testing cell viability and with PGF (0.1, 1 and 10 μM) (Figure2B) and H₂O₂ (1–100 μM) (Figure2C) for 2 h to evaluate of SOD1 mRNA expression change. Photographs showed the expression of SOD1 (240 bp) and ACTB (536 bp) mRNA. The amount of SOD1 mRNA is expressed relative to the amount of ACTB mRNA. Asterisks indicate significant differences between treated and untreated cells (P<0.05). All values represent mean ± SEM of four separate experiments.