ANOVA, analysis of variance; HDL, high-density lipoprotein; NOMAC, nomegestrol acetate; OVX, ovariectomized. N = 10 per group; * P<0.05 vs OVX control (ANOVA).
Female rats weighing 180 to 200 g were OVX or kept intact 1 week before treatment was started. Rats (n = 10) were orally treated with vehicle or 10 mg/kg/day of NOMAC for 3 weeks. Blood samples were collected from fasting animals from the retro-orbital venous sinus under light ether anesthesia 1 hour ±30 minutes after treatment. Plasma samples were stored at –20ºC until measuring the alkaline phosphatase, glucose, insulin, triglycerides, cholesterol, and HDL cholesterol.