Testicular cross-sections of 64 day-old control (Figure2A) and doxorubicin-treated (Figures2B-D) rats submitted to the PAS + H method. The Figure 2A depicts part of a tubular section containing Sertoli cell nuclei with normal morphology. These nuclei are located close to the basal membrane (arrows) of the seminiferous epithelium and show evident nucleolus (inset; arrowhead). In the Figure 2B, a Sertoli cell only tubular section is observed. In this tubular section, one of the Sertoli cell nuclei is far from the basal membrane (arrowhead) and another is sloughed into the tubular lumen (arrow). The Figure 2C depicts a sloughed portion of seminiferous epithelium (arrowheads) into the tubular lumen in which a Sertoli cell nucleus can be seen (arrow). In the inset, this Sertoli cell nucleus with irregular profile shows abnormal clear areas (arrow). Figure 2D shows a tubular section in stage IX of the seminiferous epithelium cycle with retention of step 19 spermatids (arrow). Note the presence of intraepithelial spaces and free primary spermatocytes associated.