Testicular cross-sections of 40 day-old control (1A) and doxorubicin-treated (1B-1D) rats submitted to PAS + H histochemical method. The Figure 1A shows seminiferous epithelium presenting normal morphology. The nuclei of Sertoli cells show evident nucleoli and are located close to the basal membrane of the seminiferous epithelium (inset; arrowheads). In the Figure 1B, a tubular section showing germ cell depletion is observed. Figure 1C depicts a detached portion of seminiferous epithelium in which Sertoli cell nuclei are observed (arrows). The Figure 1D shows Sertoli cell nuclei into the tubular lumen (arrows).