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Table 3 Treatment parameters for Study 2

From: Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system

Group name Untreated 37C
2 × 15
saline
37C
2 × 10
saline
37C
1 × 10
35C
2 × 15
saline
35C
2 × 15
water
35C
2 × 10
saline
2 W/cm2
Group number 8 9 10 11 12 13 14
Coupling medium (°C) NR 37 37 37 35 35 35
Treatments - 2 2 1 2 2 2
Duration (minutes) - 15 10 10 15 15 10
Coupling medium - dg-Na+ dg-Na+ dg-Na+
or DW
dg-Na+ dg-DW dg-Na+
Intensity (W/cm2) - 2.2 2.2 2.2 2.2 2.2 2.0
Frequency (MHz) - 3 3 3 3 3 3
Transducer (cm2) - 5 5 5 5 5 5
Rotation - + + + + + +
Animals 2 7 4 5 8 4 4
  1. Degassed, 3% sodium chloride was used as the coupling medium (dg-Na+), otherwise distilled water (DW) or degassed, distilled water was used (dg-DW). Temperature of the coupling medium was noted, otherwise there was no regulation (NR). Transducer ME7413 had a surface area of ~ 5 cm2 (5). Transducers were stationary (-) or were rotated in a plane parallel to the bottom of the ultrasound chamber (+). All groups received two consecutive treatments separated by two days, except as noted for Groups 8 and 11.