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Table 2 Effect of maternal obesity and vitrification on embryo development on days 2–5 of culture

From: Obesity does not aggravate vitrification injury in mouse embryos: a prospective study

 

Fresh

Vitrified

P a

Normal weight

Obese

Normal weight

Obese

No. of zygotes

30.9 ± 1.9

30.3 ± 1.3

-

-

NS

Cleavage rate (%)

64.3 ± 5.2

61.2 ± 4.5

-

-

NS

Six–eight-cell ratio (%)

75.1 ± 5.3

62.4 ± 4.2

-

-

<0.05

Morula (%)

     

Day 4

19.5b

41.6c

34.2

42.2

<0.01

Day 5

5.7

9.1

10.6

10.8

NS

≥Early blastocyst (%)

     

Day 4

40.2

32.5

36.8

38.5

NS

Day 5

39.1

49.4

42.1

39.8

NS

≥Expanded blastocyst (%)

     

Day 4

35.7b

18.1c

25.0

16.9

<0.01

Day 5

48.3b

24.6c

36.8

32.5

<0.01

Degeneration rate (%)

     

Day 4

4.6

7.8

3.9

2.4

NS

Day 5

6.9b

16.9c

10.5

16.9

<0.01

  1. Notes: Data represent n = 10 mice/group (from 630 and 609 total zygotes in normal-weight and obese females, respectively). Cleavage rate = number of cleaved embryos on day 2/total number of one-cell embryos recovered. Six–eight-cell ratio = mean number of embryos scored morphologically as having six–eight cells/mean number of cleaved embryos on day 2. Day 4 and 5 data are expressed as mean number of embryos at each stage/mean number of six–eight-cell embryos on day 3 (fresh) or total number of thawed embryos (vitrified). Degeneration rate = number of embryos that have ceased development/number of six–eight-cell embryos on day 3 (fresh) or number of thawed embryos (vitrified). ≥Early blastocyst = early-, mid-, or late-stage blastocyst. ≥Expanded blastocyst = expanded, hatching, or hatched blastocyst. NS = not significant; aChi-squared test; bvs.c, P<0.01.
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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