Table 6 Details of primers used for RT-PCR
From: In vitro and in vivodevelopment of mice morulae after storage in non-frozen conditions
Gen symbol | MGI official name | Primers sequence (5′– 3′) | Fragment size | Gene bank accesion n° |
|---|---|---|---|---|
Bax | BCL2-associated X protein | 5′ CTACTTTGCCAGCAAACTGG | 159 | NM_007527.3 |
3′ TCCCAAAGTAGGAGAGGA | ||||
Bcl2 | BCL2-like 1 | 5′ GGAGCTGGTGGTTGACTTTC | 517 | NM_009743.4| |
3′ CTAGGTGGTCATTCAGGTAAG | ||||
ErV4 | Murine endogenous retovirus-L | 5′ TGCTTGGGCTCAGCAACATGG | 278 | XM_001478088.1| |
3′ GACAGAATGCCTCATCTATCGT | ||||
Iap | Intracisternal-A particle | 5′ GGGTATTGTTGAGCGTGCGC | 333 | XM_001477167.1| |
3′ TCGGGTGAGTCTTTCTGGTAC | ||||
Terf1 | Telomeric repeat binding factor 1 | 5′ TTCAACAACCGAACAAGTGTC | 215 | Mm 4306 |
3′TCTCTTTCTCTTCCCCCTCC | ||||
Cx43 (Gaj1) | Gap junction membrane channel protein alpha 1 | 5′TACCACGCCACCACTGGCCCA | 290 | Mm 4504 |
3′ATTCTGGTTGTCGTCGGGGAAATC | ||||
Nanog | Nanog homebox | 5′AGGGTCTGCTACTGAGATGCTCTG | 363 | Mm 6047 |
3′CAACCACTGGTTTTTCTGCCACCG | ||||
Oct3/4 (Pou5f1) | POU domain, class 5, transcription factor 1 | 5′GGAGAGGTGAAACCGTCCCTAGG | 312 | Mm 17031 |
3′AGAGGAGGTTCCCTCTGAGTTGC | ||||
Gapdh | Glyceraldehyde-3-Phosphate dehydrogenase | 5′GGGTGTGAACCACGAGAAATATGA | 250 | Mm 379644 |
3′CCTTCCACAATGCCAAAGT | ||||
H2afz | Histone H2az | 5′TGTGTACAGCGCAGCCATCCTG | 208 | NM_016750.2 |
3′CTTCCCGATCAGCGATTTGTGG |