Analysis of mRNA transcription levels for developmentally important genes. Genes assayed were: A) apoptosis (Bax, Bcl2), B) cell-to-cell adhesion (Gaj1), and methabolism (Gapdh) C) totipotency (Pou5f1, Nanog) D) retrotransposon expression (Erv4 and Iap) and telomere extension (Terf1), after storage for 48 hours in KSOM and KSOMh at 4°C, 15°C and 37°C. Mean ± SEM. Significant differences were denoted by different letters. ANOVA analysis was performed.