|
Treatment groups
|
Day, time & number of females ovipositing
|
Oviposition (%)*
|
|---|
|
Day
|
1
|
2
|
3
|
4
|
5
|
6
| |
|
Light/dark (24 hr)**
|
D
|
L
|
L
|
D
|
D
|
L
|
L
|
D
|
D
|
L
|
L
|
D
|
D
|
L
|
L
|
D
|
D
|
L
|
L
|
D
|
D
| |
|
(1) Control – Saline only: 4 injections
|
|
Saline
| | | | | | | | | | | | | | | | | | | | | |
0/6 (0%)
|
|
(2) hCG only
|
|
hCG 900
| | | | | | | | |
6
| | | | | | | | | | | | |
6/20 (30%)
|
|
hCG 1050-1400
| | | | | | | | |
1
| | | |
1
|
1
|
1
| | | | | | |
4/17 (23.5%)
|
|
All hCG only
| |
10/37 (27%)
|
|
(3) hCG and pituitary glands
|
|
hCG 1200–1500 + 6 pituitaries
| | | | | | | | |
1
|
1
| | | | | | | | | | | |
2/7 (29%)
|
|
(4) PMSG and hCG (200 IU***)
|
|
50 PMSG; -3 days
| | | | | |
1
| | | | | | | | | | | | | | | |
1/6 (17%)
|
|
75 PMSG;-4, -2 days****
| | | | | | | | | |
4
| | | | | | | | | | | |
4/12 (33%)
|
|
75 PMSG;-5, -3 days****
| | | | | | | | |
6
|
6
| |
1
|
6
| | | |
1
| | | |
1
|
21/41 (51%)
|
|
75 PMSG;-6, -4 days****
| | | | |
1
| | |
1
|
1
| | | |
1
|
1
| | |
1
| | | | |
6/9 (67%)
|
|
All PMSG and hCG (200 IU)
| |
32/68 (47%)
|
- Oviposition by female M. fasciolatus after either (1) sham injections with saline (2) injections with human chorionic gonadotropin (hCG) only (3) injections with human gonadotropin and 6 amphibian pituitary glands (B. marinus) (4) injection with pregnant mare serum gonadotropin (PMSG) indicated number of days before administration of first of two doses of human chorionic gonadotropin. * Number ovipositing/Number treated (% ovipositing) ** Light/Dark cycle = 12 hr L: 12 hr D; each L or D represents 6 hrs ***hCG = 100 IU administered on each of days 1 and 2. **** Where PMSG total dose = 75 IU: first dose = 50 IU; second dose = 25 IU administered on the indicated number of days before the first dose of hCG.
