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Table 1 Published protocols for the induction of oviposition in Xenopus

From: Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the Barred Frog Mixophyes fasciolatus (Myobatrachidae)

Species PMSG priming dose (FSH activity)* hCG priming dose (LH activity)* “Ovulatory” hCG (dose or source) on Day 1** Oviposition*** References
Xenopus laevis    Human pregnant urine Time not stated [2, 10]
X. laevis    90 IU Time not stated [11]
X. laevis    100-200 IU (< 100 g bwt) + 6–12 hrs [12]
200–600 IU (> 100 g bwt)
X. laevis    300 IU Time not stated [13]
X. laevis    500 IU + 8–10 hrs [14]
X. laevis    500 IU Time not stated [15]
X. laevis    500-700 IU Day 1 [16]
X. laevis    750 IU + 8–12 hrs [17]
X. laevis    900 IU + 8–10 hrs [18]
X. laevis    1000 IU + 10 hrs [19]
X. laevis    1000 IU + 18 hrs [20]
X. laevis   50 IU, Day −14 to −5 (early ovulation if priming after day −5; priming effective up to 1 month) 500-800 IU + 9–14 hrs [21]
X. laevis   50-200 IU, Day 1 (−8 hrs) 300-500 IU; Day 1 (0 hrs) + 8 hrs [22]
X. laevis 40 IU, Day −4 to −3   250-500 IU + 6–8 hrs [23]
X. laevis 50 IU, Day?   700 IU Time not stated [24]
X. laevis 50 IU, Day −5 to −3   500 IU + 12–14 hrs [25]
X. laevis 50 IU, Day −17
25 IU, Day −14
  500 IU (hCG inducible over 14 days from second PMSG) + 16–18 hrs [26]
X. laevis 100 IU, Day −1   500 IU Day 2, avg 68% [27]
X. tropicalis   20 IU, Day −2 100 IU Natural pairing [28]
X. tropicalis 15 IU, Day −4 to −3   150 IU + 4 hrs [29]
  1. Protocols for the induction of oviposition of Xenopus (X. laevis and X. (Silurana) tropicalis) utilising hCG with, or without, priming with mammalian gonadotropins with FSH or LH activity. * Day of administration of priming dose of PMSG or hCG expressed as days before the main “ovulatory” hCG dose (expressed as negative values). ** Day of administration of “ovulatory” hCG dose expressed as Day 1 to facilitate comparison between studies listed in Tables 1 and 2; hCG administered as either reconstituted or synthetic hCG (dose in IU) or as direct injection of pregnant human urine (dose unknown) *** Time or day of oviposition (generally, hours after the main “ovulatory” dose of hCG on Day 1). Species names are from original papers, however, Xenopus tropicalis is now known as Silurana tropicalis.