Figure 5
Effect of c-src siRNA on the activation of primordial follicle growth. A, Ovaries were cultured for 8 days with the indicated treatments: blank control, negative control, 100 μmol/L siRNA targeting c-src. Ovarian sections were stained with hematoxylin and eosin to show the follicles at different developmental stages. Scale bar: 20 μm. HE staining X400 B, The number of follicles was counted in serial cross-sections. The percentage of the number of each category over the total number was plotted. The data are presented as the means ± SEM. Bars with different superscripts are statistically different (P<0.01). Blank control corresponds to the group with c-src-non-targeting oligonucleotides, the negative control corresponds to the group with blank vector (lentivirus without siRNA), and c-src siRNA corresponds to the group with lentivirus packaging c-src siRNA. The data are presented as the means ± SEM (n = 3). **P<0.01 compared with negative control group.