Figure 3

A representative picture showing the total numbers of cells examined under fluorescence (FITC, 512 nm) for superoxide dismutase 1 (SOD1). Day 7 bovine blastocysts derived from embryos exposed to BoHV-5 infection (A-C) and embryos that were unexposed (D-E) showing marked to mild intensity of SOD1-positive signals; F) electrophoresis agarose gel of SOD1 RT-PCR: MW-molecular weight 1-kb plus; lane 1 represents RT-PCR results from pure water used as a negative control; lane 2 shows the negative RT-PCR amplification of 7 d unexposed embryos; lane 3 shows the positive RT-PCR amplification of exposed embryos (297 bp). Scale bar is equal to 100 μm.