Figure 2

Day 7 in vitro cultured bovine blastocysts derived from oocytes exposed and unexposed to BoHV-5. MitoTracker Green FM labeling under fluorescence (500 nm) demonstrates an intense signal inner cell mass of exposed embryos; Jc-1 labeling under fluorescence (515–488 nm) reveals an intense signal in exposed embryos; Hoechst 33342 staining under fluorescence (488 nm) illustrates no difference between the qualities of exposed and unexposed embryos. Scale bar is equal to 20 μm.