Figure 3

Induction of BNC-specific genes during the on-Matrigel culturing of BT cell lines. The BNC-specific gene expression levels of several BT cell lines, including BT-1, BT-C, and BT-K cells, cultured on Matrigel or collagen gel for 8 days. The mRNA expression levels of bCSH1, bPRP-1, and bPAG1 (BNC-specific genes) were normalized to GAPDH expression, and those of IFNT (an MNC-specific gene) were compared with those of the control culture (collagen-coated). The black bars, gray bars, and white bars represent the control, on-collagen gel, and on-Matrigel cultures, respectively. The asterisks indicate a significant difference compared with the control (P < 0.05).