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Table 2 Oligonucleotide primers used for real-time PCR

From: Prostaglandin receptors EP and FP are regulated by estradiol and progesterone in the uterus of ovariectomized rats

Gene Accession No. Primers
F = forward; R = reverse
Product Size Annealing Temp.
EP1 NM_013100.1 F: 5'-AGCCCCCTGCTGGTATTGGT-3'
R: 5'-AGGATCTGGTTCCACGACGCGA-3'
107 63
EP2 NM_031088.1 F: 5'-GCTCCCTGCCTTTCACAATCTTTGC-3'
R: 5'-TGGAGCTTCCGGTGCTCTCAGT-3'
200 60
EP3 NM_012704.1 F: 5'-ACTGTCCGTCTGCTGGTCGC-3'
R: 5'-TGGTTCAGCGAAGCCAGGCG-3'
147 64
EP4 NM_032076.3 F: 5'-ATTCCGCTCGTGGTGCGAGTGT-3'
R: 5'-CACCAATGCGGCAGAAGAGGCA-3'
202 60
FP NM_013115.1 F: 5'-TCAGCAGCACAGGCAAGGCA-3'
R: 5'-TGGCCATCGTCACCAGAAAGGGA-3'
110 64
Rplp0 NM_001002.3 F: 5'-GGCGACCTGGAAGTCCAACT-3'
R: 5'-CCATCAGCACCACAGCCTTC-3'
149 57