Table 2 Oligonucleotide primers used for real-time PCR
Gene | Accession No. | Primers F = forward; R = reverse | Product Size | Annealing Temp. |
|---|---|---|---|---|
EP1 | NM_013100.1 | F: 5'-AGCCCCCTGCTGGTATTGGT-3' R: 5'-AGGATCTGGTTCCACGACGCGA-3' | 107 | 63 |
EP2 | NM_031088.1 | F: 5'-GCTCCCTGCCTTTCACAATCTTTGC-3' R: 5'-TGGAGCTTCCGGTGCTCTCAGT-3' | 200 | 60 |
EP3 | NM_012704.1 | F: 5'-ACTGTCCGTCTGCTGGTCGC-3' R: 5'-TGGTTCAGCGAAGCCAGGCG-3' | 147 | 64 |
EP4 | NM_032076.3 | F: 5'-ATTCCGCTCGTGGTGCGAGTGT-3' R: 5'-CACCAATGCGGCAGAAGAGGCA-3' | 202 | 60 |
FP | NM_013115.1 | F: 5'-TCAGCAGCACAGGCAAGGCA-3' R: 5'-TGGCCATCGTCACCAGAAAGGGA-3' | 110 | 64 |
Rplp0 | NM_001002.3 | F: 5'-GGCGACCTGGAAGTCCAACT-3' R: 5'-CCATCAGCACCACAGCCTTC-3' | 149 | 57 |